1cqp

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[[Image:1cqp.jpg|left|200px]]<br /><applet load="1cqp" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1cqp, resolution 2.60&Aring;" />
 
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'''CRYSTAL STRUCTURE ANALYSIS OF THE COMPLEX LFA-1 (CD11A) I-DOMAIN / LOVASTATIN AT 2.6 A RESOLUTION'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE ANALYSIS OF THE COMPLEX LFA-1 (CD11A) I-DOMAIN / LOVASTATIN AT 2.6 A RESOLUTION==
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The lymphocyte function-associated antigen (LFA-1) belongs to the family, of beta2-integrins and plays an important role in T-cell activation and, leukocyte migration to sites of inflammation. We report here that, lovastatin, a drug clinically used for lowering cholesterol levels, inhibits the interaction of human LFA-1 with its counter-receptor, intercellular adhesion molecule-1. Using nuclear magnetic resonance, spectroscopy and X-ray crystallography we show that the inhibitor binds to, a highly conserved domain of the LFA-1 alpha-chain called the I-domain., The first three-dimensional structure of an integrin inhibitor bound to, its receptor reveals atomic details for a hitherto unknown mode of LFA-1, inhibition. It also sheds light into possible mechanisms of LFA-1 mediated, signalling and will support the design of novel anti-adhesive and, immunosuppressive drugs.
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<StructureSection load='1cqp' size='340' side='right'caption='[[1cqp]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1cqp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CQP FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=803:LOVASTATIN'>803</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cqp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cqp OCA], [https://pdbe.org/1cqp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cqp RCSB], [https://www.ebi.ac.uk/pdbsum/1cqp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cqp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ITAL_HUMAN ITAL_HUMAN] Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cq/1cqp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cqp ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1CQP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=803:'>803</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQP OCA].
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*[[Integrin 3D structures|Integrin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural basis for LFA-1 inhibition upon lovastatin binding to the CD11a I-domain., Kallen J, Welzenbach K, Ramage P, Geyl D, Kriwacki R, Legge G, Cottens S, Weitz-Schmidt G, Hommel U, J Mol Biol. 1999 Sep 10;292(1):1-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10493852 10493852]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Cottens, S.]]
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[[Category: Cottens S]]
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[[Category: Geyl, D.]]
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[[Category: Geyl D]]
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[[Category: Hommel, U.]]
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[[Category: Hommel U]]
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[[Category: Kallen, J.]]
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[[Category: Kallen J]]
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[[Category: Kriwacki, R.]]
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[[Category: Kriwacki R]]
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[[Category: Legge, G.]]
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[[Category: Legge G]]
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[[Category: Ramage, P.]]
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[[Category: Ramage P]]
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[[Category: Weitz-Schmidt, G.]]
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[[Category: Weitz-Schmidt G]]
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[[Category: Welzenbach, K.]]
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[[Category: Welzenbach K]]
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[[Category: 803]]
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[[Category: MG]]
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[[Category: rossman fold]]
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[[Category: structural basis for lfa-1 inhibition]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:36:45 2008''
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Current revision

CRYSTAL STRUCTURE ANALYSIS OF THE COMPLEX LFA-1 (CD11A) I-DOMAIN / LOVASTATIN AT 2.6 A RESOLUTION

PDB ID 1cqp

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