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Anthrax protective antigen

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{{STRUCTURE_1t6b| PDB=1t6b | SIZE=400| SCENE= |right|CAPTION=Anthrax protective antigen complex with anthrax toxin receptor domain A/I, tetraethylene glycol, Ca+2, Mn+2 and Na+ ions [[1t6b]] }}
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<StructureSection load='1acc' size='350' side='right' caption='Anthrax protective antigen (PA83) complex with Ca+2 ions (PDB code [[1acc]])' scene='48/485625/Cv/8'>
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__TOC__
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== Function ==
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'''Anthrax protective antigen''' (PA) is the cell-binding part of the ''Bacillus anthracis'' anthrax toxin. The anthrax toxin is composed of the PA, lethal factor and edema factor. The PA translocates the [[Anthrax Lethal Factor|lethal]] (LF) and [[Anthrax edema factor|edema]] factors (EF) into the cell. The 83kD form of PA '''(PA83)''' binds to the anthrax toxin receptor. A 20kD fragment (PA20) is cleaved off the PA83 and the remaining '''PA63''' assembles into ring-shaped oligomer which becomes a membrane pre-channel. The PA pre-channel binds the lethal and edema factors allowing them to penetrate the membrane via pH gradient.
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'''Anthrax protective antigen''' (PA) is the cell-binding part of the ''Bacillus anthracis'' anthrax toxin. The anthrax toxin is composed of the PA, lethal factor and edema factor. The PA translocates the lethal and edema factors into the cell. The 83kD form of PA (PA83) binds to the anthrax toxin receptor. A 20kD fragment is cleaved off the PA83 and the remaining PA63 assembles into ring-shaped oligomer which becomes a membrane pre-channel. PA domain 4 is important for receptor recognition and pore formation. The PA pre-channel binds the lethal and edema factors allowing them to penetrate the membrane via pH gradient.
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'''PA83'''
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For more details see
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[[1acc]], [[3tew]], [[3q8a]], [[3q8b]] – PA<br />
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*[[Molecular Playground/Protective Antigen]]<br />
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[[3tex]], [[3tey]], [[3tez]], [[3q8c]], [[3q8e]] – PA (mutant)<br />
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*[[Anthrax Toxin Protein]]<br />
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[[3mhz]], [[3q8f]] – PA with fluoro-histidine<br />
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*[[Toxins]].
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[[1t6b]] – PA + anthrax toxin receptor A/I domain
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'''PA63'''
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== Structural highlights ==
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[[3hvd]] – PA63 (mutant)<br />
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PA contains 4 domains:<br />
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[[1tzo]] – PA63<br />
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* <scene name='48/485625/Cv/3'>Domain 1</scene> is the N terminal (in yellow). It contains the furin protease cleavage site enabling the release of PA20.<br />
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[[1tzn]] – PA63 + anthrax toxin receptor VWA domain<br />
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* <scene name='48/485625/Cv/4'>Domain 2</scene> (in cyan) is the heptamerization domain of PA63.<br />
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[[3kwv]] – PA63 (mutant) + lethal factor
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* <scene name='48/485625/Cv/5'>Domain 3</scene> (in salmon) forms the heptameric pore with domain 2 and enabling LF and EF to penetrate the cell.<br />
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* <scene name='48/485625/Cv/6'>Domain 4</scene> (in magenta) is important for receptor recognition and pore formation. <br />
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<scene name='48/485625/Cv/11'>Click here to see Ca binding site</scene>. <ref>PMID:9039918</ref>
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'''PA domain 4'''
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==3D structures of anthrax protective antigen==
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[[Anthrax protective antigen 3D structures]]
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[[3ino]] – PA domain 4<br />
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</StructureSection>
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[[3etb]] – PA domain 4 + antibody (mutant)
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== References ==
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<references/>
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[[Category:Topic Page]]

Current revision

Anthrax protective antigen (PA83) complex with Ca+2 ions (PDB code 1acc)

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References

  1. ↑ Petosa C, Collier RJ, Klimpel KR, Leppla SH, Liddington RC. Crystal structure of the anthrax toxin protective antigen. Nature. 1997 Feb 27;385(6619):833-8. PMID:9039918 doi:10.1038/385833a0

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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