1d7q

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[[Image:1d7q.jpg|left|200px]]<br /><applet load="1d7q" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1d7q" />
 
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'''HUMAN TRANSLATION INITIATION FACTOR EIF1A'''<br />
 
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==Overview==
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==HUMAN TRANSLATION INITIATION FACTOR EIF1A==
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The translation initiation factor eIF1A is necessary for directing the 43S, preinitiation complex from the 5' end of the mRNA to the initiation codon, in a process termed scanning. We have determined the solution structure of, human eIF1A, which reveals an oligonucleotide-binding (OB) fold and an, additional domain. NMR titration experiments showed that eIF1A binds, single-stranded RNA oligonucleotides in a site-specific, but, non-sequence-specific manner, hinting at an mRNA interaction rather than, specific rRNA or tRNA binding. The RNA binding surface extends over a, large area covering the canonical OB fold binding site as well as a groove, leading to the second domain. Site-directed mutations at multiple, positions along the RNA-binding surface were defective in the ability to, properly assemble preinitiation complexes at the AUG codon in vitro.
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<StructureSection load='1d7q' size='340' side='right'caption='[[1d7q]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1d7q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D7Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D7Q FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d7q OCA], [https://pdbe.org/1d7q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d7q RCSB], [https://www.ebi.ac.uk/pdbsum/1d7q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d7q ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IF1AX_HUMAN IF1AX_HUMAN] Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d7/1d7q_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d7q ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The translation initiation factor eIF1A is necessary for directing the 43S preinitiation complex from the 5' end of the mRNA to the initiation codon in a process termed scanning. We have determined the solution structure of human eIF1A, which reveals an oligonucleotide-binding (OB) fold and an additional domain. NMR titration experiments showed that eIF1A binds single-stranded RNA oligonucleotides in a site-specific, but non-sequence-specific manner, hinting at an mRNA interaction rather than specific rRNA or tRNA binding. The RNA binding surface extends over a large area covering the canonical OB fold binding site as well as a groove leading to the second domain. Site-directed mutations at multiple positions along the RNA-binding surface were defective in the ability to properly assemble preinitiation complexes at the AUG codon in vitro.
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==About this Structure==
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The eIF1A solution structure reveals a large RNA-binding surface important for scanning function.,Battiste JL, Pestova TV, Hellen CU, Wagner G Mol Cell. 2000 Jan;5(1):109-19. PMID:10678173<ref>PMID:10678173</ref>
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1D7Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D7Q OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The eIF1A solution structure reveals a large RNA-binding surface important for scanning function., Battiste JL, Pestova TV, Hellen CU, Wagner G, Mol Cell. 2000 Jan;5(1):109-19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10678173 10678173]
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</div>
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[[Category: Homo sapiens]]
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<div class="pdbe-citations 1d7q" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Battiste, J.L.]]
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[[Category: Hellen, C.U.T.]]
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[[Category: Pestova, T.V.]]
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[[Category: Wagner, G.]]
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[[Category: beta-barrel]]
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[[Category: ob-fold]]
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[[Category: rna-binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:38:55 2008''
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==See Also==
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*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Battiste JL]]
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[[Category: Hellen CUT]]
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[[Category: Pestova TV]]
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[[Category: Wagner G]]

Current revision

HUMAN TRANSLATION INITIATION FACTOR EIF1A

PDB ID 1d7q

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