3v0a

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[[Image:3v0a.jpg|left|200px]]
 
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==2.7 angstrom crystal structure of BoNT/Ai in complex with NTNHA==
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The line below this paragraph, containing "STRUCTURE_3v0a", creates the "Structure Box" on the page.
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<StructureSection load='3v0a' size='340' side='right'caption='[[3v0a]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3v0a]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V0A FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.703&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_3v0a| PDB=3v0a | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v0a OCA], [https://pdbe.org/3v0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v0a RCSB], [https://www.ebi.ac.uk/pdbsum/3v0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v0a ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BXAN_CLOBO BXAN_CLOBO] Assembles with botulinum neurotoxin type A (BoNT/A) and protects it against pH-mediated inactivation or protease activity at pH 2.6 (the pH of the animal gastrointestinal tract) but not at pH 6.0 (PubMed:22363010). Necessary for neurotoxicity.<ref>PMID:22363010</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Botulinum neurotoxins (BoNTs) are highly poisonous substances that are also effective medicines. Accidental BoNT poisoning often occurs through ingestion of Clostridium botulinum-contaminated food. Here, we present the crystal structure of a BoNT in complex with a clostridial nontoxic nonhemagglutinin (NTNHA) protein at 2.7 angstroms. Biochemical and functional studies show that NTNHA provides large and multivalent binding interfaces to protect BoNT from gastrointestinal degradation. Moreover, the structure highlights key residues in BoNT that regulate complex assembly in a pH-dependent manner. Collectively, our findings define the molecular mechanisms by which NTNHA shields BoNT in the hostile gastrointestinal environment and releases it upon entry into the circulation. These results will assist in the design of small molecules for inhibiting oral BoNT intoxication and of delivery vehicles for oral administration of biologics.
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===2.7 angstrom crystal structure of BoNT/Ai in complex with NTNHA===
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Botulinum neurotoxin is shielded by NTNHA in an interlocked complex.,Gu S, Rumpel S, Zhou J, Strotmeier J, Bigalke H, Perry K, Shoemaker CB, Rummel A, Jin R Science. 2012 Feb 24;335(6071):977-81. PMID:22363010<ref>PMID:22363010</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3v0a" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Antibody 3D structures|Antibody 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 22363010 is the PubMed ID number.
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*[[Botulinum neurotoxin 3D structures|Botulinum neurotoxin 3D structures]]
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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{{ABSTRACT_PUBMED_22363010}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3v0a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum] and [http://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V0A OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:022363010</ref><references group="xtra"/>
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[[Category: Clostridium botulinum]]
[[Category: Clostridium botulinum]]
[[Category: Lama glama]]
[[Category: Lama glama]]
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[[Category: Bigalke, H.]]
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[[Category: Large Structures]]
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[[Category: Gu, S.]]
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[[Category: Bigalke H]]
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[[Category: Jin, R.]]
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[[Category: Gu S]]
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[[Category: Perry, K.]]
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[[Category: Jin R]]
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[[Category: Rummel, A.]]
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[[Category: Perry K]]
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[[Category: Rumpel, S.]]
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[[Category: Rummel A]]
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[[Category: Shoemaker, C B.]]
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[[Category: Rumpel S]]
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[[Category: Strotmeier, J.]]
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[[Category: Shoemaker CB]]
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[[Category: Zhou, J.]]
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[[Category: Strotmeier J]]
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[[Category: Botulinum neurotoxin]]
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[[Category: Zhou J]]
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[[Category: Neurotoxin associated protein]]
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[[Category: Ntnha]]
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[[Category: Progenitor toxin complex]]
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[[Category: Toxin]]
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[[Category: Vhh bound interlocked complex]]
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Current revision

2.7 angstrom crystal structure of BoNT/Ai in complex with NTNHA

PDB ID 3v0a

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