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3v0b

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[[Image:3v0b.jpg|left|200px]]
 
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==3.9 angstrom crystal structure of BoNT/Ai in complex with NTNHA==
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The line below this paragraph, containing "STRUCTURE_3v0b", creates the "Structure Box" on the page.
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<StructureSection load='3v0b' size='340' side='right'caption='[[3v0b]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3v0b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V0B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V0B FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bont/a, boNT/A, bonta ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896]), ant, ntnh ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>
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{{STRUCTURE_3v0b| PDB=3v0b | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v0b OCA], [https://pdbe.org/3v0b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v0b RCSB], [https://www.ebi.ac.uk/pdbsum/3v0b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v0b ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Botulinum neurotoxins (BoNTs) are highly poisonous substances that are also effective medicines. Accidental BoNT poisoning often occurs through ingestion of Clostridium botulinum-contaminated food. Here, we present the crystal structure of a BoNT in complex with a clostridial nontoxic nonhemagglutinin (NTNHA) protein at 2.7 angstroms. Biochemical and functional studies show that NTNHA provides large and multivalent binding interfaces to protect BoNT from gastrointestinal degradation. Moreover, the structure highlights key residues in BoNT that regulate complex assembly in a pH-dependent manner. Collectively, our findings define the molecular mechanisms by which NTNHA shields BoNT in the hostile gastrointestinal environment and releases it upon entry into the circulation. These results will assist in the design of small molecules for inhibiting oral BoNT intoxication and of delivery vehicles for oral administration of biologics.
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===3.9 angstrom crystal structure of BoNT/Ai in complex with NTNHA===
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Botulinum neurotoxin is shielded by NTNHA in an interlocked complex.,Gu S, Rumpel S, Zhou J, Strotmeier J, Bigalke H, Perry K, Shoemaker CB, Rummel A, Jin R Science. 2012 Feb 24;335(6071):977-81. PMID:22363010<ref>PMID:22363010</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3v0b" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Botulinum neurotoxin 3D structures|Botulinum neurotoxin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 22363010 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_22363010}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Bacillus botulinus van ermengem 1896]]
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[[3v0b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V0B OCA].
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[[Category: Large Structures]]
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[[Category: Bigalke, H]]
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==Reference==
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[[Category: Gu, S]]
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<ref group="xtra">PMID:022363010</ref><references group="xtra"/>
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[[Category: Jin, R]]
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[[Category: Clostridium botulinum]]
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[[Category: Perry, K]]
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[[Category: Bigalke, H.]]
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[[Category: Rummel, A]]
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[[Category: Gu, S.]]
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[[Category: Rumpel, S]]
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[[Category: Jin, R.]]
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[[Category: Shoemaker, C B]]
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[[Category: Perry, K.]]
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[[Category: Strotmeier, J]]
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[[Category: Rummel, A.]]
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[[Category: Zhou, J]]
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[[Category: Rumpel, S.]]
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[[Category: Shoemaker, C B.]]
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[[Category: Strotmeier, J.]]
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[[Category: Zhou, J.]]
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[[Category: Botulinum neurotoxin]]
[[Category: Botulinum neurotoxin]]
[[Category: Toxin]]
[[Category: Toxin]]
[[Category: Vhh free inlocked complex]]
[[Category: Vhh free inlocked complex]]

Current revision

3.9 angstrom crystal structure of BoNT/Ai in complex with NTNHA

PDB ID 3v0b

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