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1egi

From Proteopedia

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[[Image:1egi.jpg|left|200px]]<br /><applet load="1egi" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1egi, resolution 2.30&Aring;" />
 
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'''STRUCTURE OF A C-TYPE CARBOHYDRATE-RECOGNITION DOMAIN (CRD-4) FROM THE MACROPHAGE MANNOSE RECEPTOR'''<br />
 
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==Overview==
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==STRUCTURE OF A C-TYPE CARBOHYDRATE-RECOGNITION DOMAIN (CRD-4) FROM THE MACROPHAGE MANNOSE RECEPTOR==
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The mannose receptor of macrophages and liver endothelium mediates, clearance of pathogenic organisms and potentially harmful glycoconjugates., The extracellular portion of the receptor includes eight C-type, carbohydrate recognition domains (CRDs), of which one, CRD-4, shows, detectable binding to monosaccharide ligands. We have determined the, crystal structure of CRD-4. Although the basic C-type lectin fold is, preserved, a loop extends away from the core of the domain to form a, domain-swapped dimer in the crystal. Of the two Ca(2+) sites, only the, principal site known to mediate carbohydrate binding in other C-type, lectins is occupied. This site is altered in a way that makes sugar, binding impossible in the mode observed in other C-type lectins. The, structure is likely to represent an endosomal form of the domain formed, when Ca(2+) is lost from the auxiliary calcium site. The structure, suggests a mechanism for endosomal ligand release in which the auxiliary, calcium site serves as a pH sensor. Acid pH-induced removal of this Ca(2+), results in conformational rearrangements of the receptor, rendering it, unable to bind carbohydrate ligands.
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<StructureSection load='1egi' size='340' side='right'caption='[[1egi]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1egi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EGI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EGI FirstGlance]. <br>
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1EGI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EGI OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1egi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1egi OCA], [https://pdbe.org/1egi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1egi RCSB], [https://www.ebi.ac.uk/pdbsum/1egi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1egi ProSAT]</span></td></tr>
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Structure of a C-type carbohydrate recognition domain from the macrophage mannose receptor., Feinberg H, Park-Snyder S, Kolatkar AR, Heise CT, Taylor ME, Weis WI, J Biol Chem. 2000 Jul 14;275(28):21539-48. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10779515 10779515]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MRC1_HUMAN MRC1_HUMAN] Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eg/1egi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1egi ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Feinberg, H.]]
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[[Category: Feinberg H]]
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[[Category: Heise, C.T.]]
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[[Category: Heise CT]]
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[[Category: Kolatkar, A.R.]]
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[[Category: Kolatkar AR]]
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[[Category: Park-Snyder, S.]]
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[[Category: Park-Snyder S]]
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[[Category: Taylor, M.E.]]
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[[Category: Taylor ME]]
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[[Category: Weis, W.I.]]
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[[Category: Weis WI]]
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[[Category: CA]]
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[[Category: c-type lectin]]
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[[Category: mannose receptor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:43:15 2008''
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Current revision

STRUCTURE OF A C-TYPE CARBOHYDRATE-RECOGNITION DOMAIN (CRD-4) FROM THE MACROPHAGE MANNOSE RECEPTOR

PDB ID 1egi

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