This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2y95

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:06, 14 June 2023) (edit) (undo)
 
(5 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2y95.png|left|200px]]
 
-
<!--
+
==Solution structure of AUCG tetraloop hairpin found in human Xist RNA A-repeats essential for X-inactivation==
-
The line below this paragraph, containing "STRUCTURE_2y95", creates the "Structure Box" on the page.
+
<StructureSection load='2y95' size='340' side='right'caption='[[2y95]]' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2y95]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y95 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y95 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y95 OCA], [https://pdbe.org/2y95 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y95 RCSB], [https://www.ebi.ac.uk/pdbsum/2y95 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y95 ProSAT]</span></td></tr>
-
-->
+
</table>
-
{{STRUCTURE_2y95| PDB=2y95 | SCENE= }}
+
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
X-chromosome inactivation (XCI) in female mammals depends on the noncoding RNA X inactivation specific transcript (Xist). The mechanism of chromosome-wide silencing by Xist is poorly understood. While it is established that the 5' region of Xist RNA, comprising the A-repeats and holding 7.5-8.5 copies of a conserved 26-mer sequence, is essential for Xist-mediated silencing, high-resolution structural information for the A-repeats is not available. Here, we report the three-dimensional solution structure of a 14-mer hairpin in the 5' region of a human A-repeat. This hairpin is remarkably stable and adopts a novel AUCG tetraloop fold, the integrity of which is required for silencing. We show that, contrary to previous predictions, the 3' region of single or tandem A-repeats mediates duplex formation in vitro. Significantly, mutations in this region disrupt the inter-repeat duplex formation in vitro and abrogate the silencing function of Xist A-repeats in vivo. Our data suggest that the complete A-repeat region may be stabilized by inter-repeat duplex formation and, as such, may provide a platform for multimerization and specific recognition of the AUCG tetraloops by trans-acting factors.
-
===Solution structure of AUCG tetraloop hairpin found in human Xist RNA A-repeats essential for X-inactivation===
+
The Xist RNA A-repeat comprises a novel AUCG tetraloop fold and a platform for multimerization.,Duszczyk MM, Wutz A, Rybin V, Sattler M RNA. 2011 Nov;17(11):1973-82. Epub 2011 Sep 26. PMID:21947263<ref>PMID:21947263</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_21947263}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 2y95" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 21947263 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_21947263}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Homo sapiens]]
-
[[2y95]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y95 OCA].
+
[[Category: Large Structures]]
-
 
+
[[Category: Duszczyk MM]]
-
==Reference==
+
[[Category: Sattler M]]
-
<ref group="xtra">PMID:021947263</ref><ref group="xtra">PMID:021779925</ref><references group="xtra"/>
+
-
[[Category: Duszczyk, M M.]]
+
-
[[Category: Sattler, M.]]
+
-
[[Category: Rna]]
+
-
[[Category: Rna stemloop]]
+
-
[[Category: X chromosome inactivation]]
+

Current revision

Solution structure of AUCG tetraloop hairpin found in human Xist RNA A-repeats essential for X-inactivation

PDB ID 2y95

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2y95"
Personal tools