2y95

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[[Image:2y95.png|left|200px]]
 
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==Solution structure of AUCG tetraloop hairpin found in human Xist RNA A-repeats essential for X-inactivation==
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The line below this paragraph, containing "STRUCTURE_2y95", creates the "Structure Box" on the page.
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<StructureSection load='2y95' size='340' side='right'caption='[[2y95]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2y95]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y95 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y95 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y95 OCA], [https://pdbe.org/2y95 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y95 RCSB], [https://www.ebi.ac.uk/pdbsum/2y95 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y95 ProSAT]</span></td></tr>
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{{STRUCTURE_2y95| PDB=2y95 | SCENE= }}
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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X-chromosome inactivation (XCI) in female mammals depends on the noncoding RNA X inactivation specific transcript (Xist). The mechanism of chromosome-wide silencing by Xist is poorly understood. While it is established that the 5' region of Xist RNA, comprising the A-repeats and holding 7.5-8.5 copies of a conserved 26-mer sequence, is essential for Xist-mediated silencing, high-resolution structural information for the A-repeats is not available. Here, we report the three-dimensional solution structure of a 14-mer hairpin in the 5' region of a human A-repeat. This hairpin is remarkably stable and adopts a novel AUCG tetraloop fold, the integrity of which is required for silencing. We show that, contrary to previous predictions, the 3' region of single or tandem A-repeats mediates duplex formation in vitro. Significantly, mutations in this region disrupt the inter-repeat duplex formation in vitro and abrogate the silencing function of Xist A-repeats in vivo. Our data suggest that the complete A-repeat region may be stabilized by inter-repeat duplex formation and, as such, may provide a platform for multimerization and specific recognition of the AUCG tetraloops by trans-acting factors.
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===Solution structure of AUCG tetraloop hairpin found in human Xist RNA A-repeats essential for X-inactivation===
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The Xist RNA A-repeat comprises a novel AUCG tetraloop fold and a platform for multimerization.,Duszczyk MM, Wutz A, Rybin V, Sattler M RNA. 2011 Nov;17(11):1973-82. Epub 2011 Sep 26. PMID:21947263<ref>PMID:21947263</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2y95" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 21947263 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21947263}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Homo sapiens]]
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[[2y95]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y95 OCA].
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[[Category: Large Structures]]
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[[Category: Duszczyk MM]]
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==Reference==
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[[Category: Sattler M]]
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<ref group="xtra">PMID:021947263</ref><ref group="xtra">PMID:021779925</ref><references group="xtra"/>
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[[Category: Duszczyk, M M.]]
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[[Category: Sattler, M.]]
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[[Category: Rna]]
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[[Category: Rna stemloop]]
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[[Category: X chromosome inactivation]]
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Current revision

Solution structure of AUCG tetraloop hairpin found in human Xist RNA A-repeats essential for X-inactivation

PDB ID 2y95

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