1xa8

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[[Image:1xa8.png|left|200px]]
 
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==Crystal Structure Analysis of Glutathione-dependent formaldehyde-activating enzyme (Gfa)==
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The line below this paragraph, containing "STRUCTURE_1xa8", creates the "Structure Box" on the page.
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<StructureSection load='1xa8' size='340' side='right'caption='[[1xa8]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1xa8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Paracoccus_denitrificans Paracoccus denitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XA8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XA8 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_1xa8| PDB=1xa8 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xa8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xa8 OCA], [https://pdbe.org/1xa8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xa8 RCSB], [https://www.ebi.ac.uk/pdbsum/1xa8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xa8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GFA_PARDE GFA_PARDE] Catalyzes the condensation of formaldehyde and glutathione to S-hydroxymethylglutathione.<ref>PMID:11741920</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xa/1xa8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xa8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structures of glutathione-dependent formaldehyde-activating enzyme (Gfa) from Paracoccus denitrificans, which catalyzes the formation of S-hydroxymethylglutathione from formaldehyde and glutathione, and its complex with glutathione (Gfa-GTT) have been determined. Gfa has a new fold with two zinc-sulfur centers, one that is structural (zinc tetracoordinated) and one catalytic (zinc apparently tricoordinated). In Gfa-GTT, the catalytic zinc is displaced due to disulfide bond formation of glutathione with one of the zinc-coordinating cysteines. Soaking crystals of Gfa-GTT with formaldehyde restores the holoenzyme. Accordingly, the displaced zinc forms a complex by scavenging formaldehyde and glutathione. The activation of formaldehyde and of glutathione in this zinc complex favors the final nucleophilic addition, followed by relocation of zinc in the catalytic site. Therefore, the structures of Gfa and Gfa-GTT draw the critical association between a dynamic zinc redox switch and a nucleophilic addition as a new facet of the redox activity of zinc-sulfur sites.
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===Crystal Structure Analysis of Glutathione-dependent formaldehyde-activating enzyme (Gfa)===
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A dynamic zinc redox switch.,Neculai AM, Neculai D, Griesinger C, Vorholt JA, Becker S J Biol Chem. 2005 Jan 28;280(4):2826-30. Epub 2004 Nov 17. PMID:15548539<ref>PMID:15548539</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15548539}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1xa8" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15548539 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15548539}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[1xa8]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Paracoccus_denitrificans Paracoccus denitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XA8 OCA].
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==Reference==
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<ref group="xtra">PMID:015548539</ref><ref group="xtra">PMID:011741920</ref><references group="xtra"/>
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[[Category: Paracoccus denitrificans]]
[[Category: Paracoccus denitrificans]]
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[[Category: Becker, S.]]
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[[Category: Becker S]]
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[[Category: Griesinger, C.]]
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[[Category: Griesinger C]]
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[[Category: Neculai, A M.]]
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[[Category: Neculai AM]]
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[[Category: Neculai, D.]]
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[[Category: Neculai D]]
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[[Category: Vorholt, J A.]]
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[[Category: Vorholt JA]]
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[[Category: Formaldehyde]]
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[[Category: Glutathione]]
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[[Category: Lyase]]
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[[Category: S-s bond]]
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Current revision

Crystal Structure Analysis of Glutathione-dependent formaldehyde-activating enzyme (Gfa)

PDB ID 1xa8

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