1g2t

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:19, 27 March 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1g2t.jpg|left|200px]]<br /><applet load="1g2t" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1g2t" />
 
-
'''SOLUTION STRUCTURE OF EOTAXIN-3'''<br />
 
-
==Overview==
+
==SOLUTION STRUCTURE OF EOTAXIN-3==
-
Eotaxin-3 is one of three related chemokines that specifically activate, chemokine receptor CCR3. We report the 3D structure and backbone dynamics, of eotaxin-3 determined by NMR spectroscopy. Eotaxin-3 is monomeric under, the conditions in this study and consists of an unstructured N-terminus, before the first two conserved cysteine residues, an irregularly, structured N-loop following the second conserved cysteine, a single turn, of 3(10)-helix, a three-stranded antiparallel beta-sheet, an alpha-helix, and an unstructured C-terminal tail. As in other chemokines, the, alpha-helix packs against one face of the beta-sheet. The average backbone, and heavy atom rmsd values of the 20 structures (residues 9-65) are 0.44, and 1.01 A, respectively. A comparison between the structures of eotaxin-3, and related chemokines suggests that the electrostatic potential in the, vicinity of a surface groove and the structure of the beta2-beta3 turn may, be important for maintaining receptor specificity. The backbone dynamics, of eotaxin-3 were determined from 15N NMR relaxation data using the, extended model free dynamics formalism. Large amplitude motions on the, picosecond to nanosecond time scale were observed in both termini and in, some residues in the N-loop, the beta1-beta2 turn, and the beta3 strand;, the location of these residues suggests a possible role for dynamics in, receptor binding and activation. In contrast to eotaxin, eotaxin-3, exhibits no substantial mobility on the microsecond to millisecond time, scale.
+
<StructureSection load='1g2t' size='340' side='right'caption='[[1g2t]]' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1g2t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G2T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G2T FirstGlance]. <br>
-
1G2T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G2T OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g2t OCA], [https://pdbe.org/1g2t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g2t RCSB], [https://www.ebi.ac.uk/pdbsum/1g2t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g2t ProSAT]</span></td></tr>
-
==Reference==
+
</table>
-
NMR solution structure and backbone dynamics of the CC chemokine eotaxin-3., Ye J, Mayer KL, Mayer MR, Stone MJ, Biochemistry. 2001 Jul 3;40(26):7820-31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11425309 11425309]
+
== Function ==
 +
[https://www.uniprot.org/uniprot/CCL26_HUMAN CCL26_HUMAN] Chemotactic for eosinophils and basophils. Binds to CCR3.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g2/1g2t_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g2t ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Mayer, K.L.]]
+
[[Category: Mayer KL]]
-
[[Category: Mayer, M.R.]]
+
[[Category: Mayer MR]]
-
[[Category: Stone, M.J.]]
+
[[Category: Stone MJ]]
-
[[Category: Ye, J.]]
+
[[Category: Ye J]]
-
[[Category: beta-beta-beta-alpha helix]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:50:35 2008''
+

Current revision

SOLUTION STRUCTURE OF EOTAXIN-3

PDB ID 1g2t

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1g2t"
Personal tools