3frh

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[[Image:3frh.png|left|200px]]
 
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==Structure of the 16S rRNA methylase RmtB, P21==
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The line below this paragraph, containing "STRUCTURE_3frh", creates the "Structure Box" on the page.
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<StructureSection load='3frh' size='340' side='right'caption='[[3frh]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3frh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The February 2012 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Aminoglycoside Antibiotics'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2012_2 10.2210/rcsb_pdb/mom_2012_2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FRH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FRH FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
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{{STRUCTURE_3frh| PDB=3frh | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3frh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3frh OCA], [https://pdbe.org/3frh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3frh RCSB], [https://www.ebi.ac.uk/pdbsum/3frh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3frh ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q763K9_ECOLX Q763K9_ECOLX]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fr/3frh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3frh ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Aminoglycosides are used extensively for the treatment of severe infections due to Gram-negative bacteria. However, certain species have become highly resistant after acquisition of genes for methyltransferases which catalyze post-transcriptional methylation of N7-G1405 in 16 S rRNA of 30 S ribosomal subunits. Inactivation of this enzymatic activity is therefore an important challenge for development of an effective therapy. The present work describes the crystallographic structures of methyltransferases RmtB and ArmA from clinical isolates. Together with biochemical experiments, the 3D structures indicate that the N-terminal domain specific for this family of methyltransferases is required for enzymatic activity. Site-directed mutagenesis has enabled important residues for catalysis and RNA binding to be identified. These high-resolution structures should underpin the design of potential inhibitors of these enzymes, which could be used to restore the activity of aminoglycosides against resistant pathogens.
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===Structure of the 16S rRNA methylase RmtB, P21===
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Structural bases for 16 S rRNA methylation catalyzed by ArmA and RmtB methyltransferases.,Schmitt E, Galimand M, Panvert M, Courvalin P, Mechulam Y J Mol Biol. 2009 May 8;388(3):570-82. Epub 2009 Mar 20. PMID:19303884<ref>PMID:19303884</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19303884}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3frh" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19303884 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19303884}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[3frh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The February 2012 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Aminoglycoside Antibiotics'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2012_2 10.2210/rcsb_pdb/mom_2012_2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FRH OCA].
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==Reference==
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<ref group="xtra">PMID:019303884</ref><references group="xtra"/>
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[[Category: Aminoglycoside Antibiotics]]
[[Category: Aminoglycoside Antibiotics]]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Large Structures]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Courvalin, P.]]
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[[Category: Courvalin P]]
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[[Category: Dupechez, M.]]
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[[Category: Dupechez M]]
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[[Category: Galimand, M.]]
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[[Category: Galimand M]]
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[[Category: Mechulam, Y.]]
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[[Category: Mechulam Y]]
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[[Category: Panvert, M.]]
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[[Category: Panvert M]]
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[[Category: Schmitt, E.]]
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[[Category: Schmitt E]]
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[[Category: Helical n-terminal domain]]
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[[Category: Methyltransferase]]
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[[Category: Methyltransferase domain]]
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[[Category: Transferase]]
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Current revision

Structure of the 16S rRNA methylase RmtB, P21

PDB ID 3frh

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OCA

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