3kzf

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[[Image:3kzf.png|left|200px]]
 
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==Structure of Giardia Carbamate Kinase==
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The line below this paragraph, containing "STRUCTURE_3kzf", creates the "Structure Box" on the page.
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<StructureSection load='3kzf' size='340' side='right'caption='[[3kzf]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3kzf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Giardia_lamblia_ATCC_50803 Giardia lamblia ATCC 50803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KZF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KZF FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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{{STRUCTURE_3kzf| PDB=3kzf | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kzf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kzf OCA], [https://pdbe.org/3kzf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kzf RCSB], [https://www.ebi.ac.uk/pdbsum/3kzf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kzf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A8BB85_GIAIC A8BB85_GIAIC]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kz/3kzf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kzf ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Carbamate kinase catalyzes the reversible conversion of carbamoyl phosphate and ADP to ATP and ammonium carbamate, which is hydrolyzed to ammonia and carbonate. The three-dimensional structure of carbamate kinase from the human parasite Giardia lamblia (glCK) has been determined at 3 A resolution. The crystals belonged to the monoclinic space group P2(1), with unit-cell parameters a = 69.77, b = 85.41, c = 102.1 A, beta = 106.8 degrees . The structure was refined to a final R factor of 0.227. The essentiality of glCK together with its absence in humans makes the enzyme an attractive candidate for anti-Giardia drug development. Steady-state kinetic rate constants have been determined. The k(cat) for ATP formation is 319 +/- 9 s(-1). The K(m) values for carbamoyl phosphate and ADP are 85 +/- 6 and 70 +/- 5 microM, respectively. The structure suggests that three invariant lysine residues (Lys131, Lys216 and Lys278) may be involved in the binding of substrates and phosphoryl transfer. The structure of glCK reveals that a glycerol molecule binds in the likely carbamoyl phosphate-binding site.
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===Structure of Giardia Carbamate Kinase===
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X-ray structure and characterization of carbamate kinase from the human parasite Giardia lamblia.,Galkin A, Kulakova L, Wu R, Nash TE, Dunaway-Mariano D, Herzberg O Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Apr 1;66(Pt 4):386-90., Epub 2010 Mar 26. PMID:20383005<ref>PMID:20383005</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20383005}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3kzf" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20383005 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20383005}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Giardia lamblia ATCC 50803]]
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[[3kzf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Giardia_lamblia_atcc_50803 Giardia lamblia atcc 50803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KZF OCA].
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[[Category: Large Structures]]
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[[Category: Galkin A]]
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==Reference==
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[[Category: Herzberg O]]
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<ref group="xtra">PMID:020383005</ref><references group="xtra"/>
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[[Category: Carbamate kinase]]
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[[Category: Giardia lamblia atcc 50803]]
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[[Category: Galkin, A.]]
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[[Category: Herzberg, O.]]
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[[Category: Arginine dihydrolase pathway]]
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[[Category: Carbamate kinase]]
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[[Category: Drug target]]
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[[Category: Giardia lamblia]]
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[[Category: Kinase]]
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[[Category: Transferase]]
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Current revision

Structure of Giardia Carbamate Kinase

PDB ID 3kzf

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