3fya

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[[Image:3fya.png|left|200px]]
 
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==Crystal Structure of an R35A mutant of the Restriction-Modification Controller Protein C.Esp1396I==
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The line below this paragraph, containing "STRUCTURE_3fya", creates the "Structure Box" on the page.
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<StructureSection load='3fya' size='340' side='right'caption='[[3fya]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3fya]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacter_sp._RFL1396 Enterobacter sp. RFL1396]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FYA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FYA FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fya FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fya OCA], [https://pdbe.org/3fya PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fya RCSB], [https://www.ebi.ac.uk/pdbsum/3fya PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fya ProSAT]</span></td></tr>
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{{STRUCTURE_3fya| PDB=3fya | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8GGH0_9ENTR Q8GGH0_9ENTR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fy/3fya_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fya ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The controller protein of the Esp1396I restriction-modification (R-M) system binds differentially to three distinct operator sequences upstream of the methyltransferase (M) and endonuclease (R) genes to regulate the timing of gene expression. The crystal structure of a complex of the protein with two adjacent operator DNA sequences has been reported; however, the structure of the free protein has not yet been determined. Here, the crystal structure of the free protein is reported, with seven dimers in the asymmetric unit. Two of the 14 monomers show an alternative conformation to the major conformer in which the side chains of residues 43-46 in the loop region flanking the DNA-recognition helix are displaced by up to 10 A. It is proposed that the adoption of these two conformational states may play a role in DNA-sequence promiscuity. The two alternative conformations are also found in the R35A mutant structure, which is otherwise identical to the native protein. Comparison of the free and bound protein structures shows a 1.4 A displacement of the recognition helices when the dimer is bound to its DNA target.
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===Crystal Structure of an R35A mutant of the Restriction-Modification Controller Protein C.Esp1396I===
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Structure of the restriction-modification controller protein C.Esp1396I.,Ball N, Streeter SD, Kneale GG, McGeehan JE Acta Crystallogr D Biol Crystallogr. 2009 Sep;65(Pt 9):900-5. Epub 2009 Aug 6. PMID:19690367<ref>PMID:19690367</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19690367}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3fya" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19690367 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19690367}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Enterobacter sp. RFL1396]]
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[[3fya]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacter_sp. Enterobacter sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FYA OCA].
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[[Category: Large Structures]]
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[[Category: Ball NJ]]
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==Reference==
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[[Category: Kneale GG]]
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<ref group="xtra">PMID:019690367</ref><ref group="xtra">PMID:018644840</ref><references group="xtra"/>
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[[Category: McGeehan JE]]
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[[Category: Enterobacter sp.]]
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[[Category: Streeter SD]]
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[[Category: Ball, N J.]]
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[[Category: Thresh SJ]]
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[[Category: Kneale, G G.]]
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[[Category: McGeehan, J E.]]
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[[Category: Streeter, S D.]]
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[[Category: Thresh, S J.]]
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[[Category: Helix-turn-helix]]
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[[Category: Restriction-modification]]
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[[Category: Transcription regulator]]
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[[Category: Transcriptional regulator]]
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Current revision

Crystal Structure of an R35A mutant of the Restriction-Modification Controller Protein C.Esp1396I

PDB ID 3fya

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