4e2h

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[[Image:4e2h.jpg|left|200px]]
 
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==Crystal structure of the periplasmic domain of Shigella flexneri WzzB==
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The line below this paragraph, containing "STRUCTURE_4e2h", creates the "Structure Box" on the page.
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<StructureSection load='4e2h' size='340' side='right'caption='[[4e2h]], [[Resolution|resolution]] 2.36&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[4e2h]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E2H FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.36&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_4e2h| PDB=4e2h | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e2h OCA], [https://pdbe.org/4e2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e2h RCSB], [https://www.ebi.ac.uk/pdbsum/4e2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e2h ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/WZZB_SHIFL WZZB_SHIFL] Confers a modal distribution of chain length on the O-antigen component of lipopolysaccharide (LPS). Gives rise to a reduced number of short chain molecules and increases in numbers of longer molecules.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The surface O-antigen polymers of gram-negative bacteria exhibit a modal length distribution that depends on dedicated chain-length regulator periplasmic proteins (Polysaccharide Co-Polymerases, PCPs) anchored in the inner membrane by two transmembrane helices. In an attempt to determine whether structural changes underlie the O-antigen modal length specification we have determined crystal structures of several closely related PCPs, namely two chimeric PCP-1 family members solved at 1.5A and 2.6A and a wild-type PCP-1 from Shigella flexneri solved at 2.8A. The chimeric proteins form circular octamers while the wild type WzzB from Shigella flexneri was found to be an open trimer. We also present the structure of a WzzBFepE mutant, which exhibits severe attenuation in its ability to produce very long O-antigen polymers. Our findings suggest that the differences in the modal length distribution depend primarily on the surface-exposed amino acids in specific regions rather than on the differences in the oligomeric state of the PCP protomers.
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===Crystal structure of the periplasmic domain of Shigella flexneri WzzB===
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Structural characterization of closely related o-antigen LPS-chain length regulators.,Kalynych S, Yao D, Magee J, Cygler M J Biol Chem. 2012 Mar 21. PMID:22437828<ref>PMID:22437828</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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[[4e2h]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E2H OCA].
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<div class="pdbe-citations 4e2h" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Shigella flexneri]]
[[Category: Shigella flexneri]]
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[[Category: Cygler, M.]]
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[[Category: Cygler M]]
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[[Category: Kalynych, S.]]
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[[Category: Kalynych S]]
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[[Category: Magee, J D.]]
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[[Category: Magee JD]]
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[[Category: Yao, D.]]
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[[Category: Yao D]]
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[[Category: Inner membrane]]
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[[Category: Lps o-antigen chain length determination]]
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[[Category: Membrane protein]]
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[[Category: Periplasmic space]]
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[[Category: Wzz]]
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Current revision

Crystal structure of the periplasmic domain of Shigella flexneri WzzB

PDB ID 4e2h

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