2ydz

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[[Image:2ydz.png|left|200px]]
 
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==X-ray structure of the cyan fluorescent protein SCFP3A (K206A mutant)==
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The line below this paragraph, containing "STRUCTURE_2ydz", creates the "Structure Box" on the page.
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<StructureSection load='2ydz' size='340' side='right'caption='[[2ydz]], [[Resolution|resolution]] 1.59&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2ydz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YDZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YDZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.59&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRF:[(4Z)-2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(1H-INDOL-3-YLMETHYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>CRF</scene></td></tr>
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{{STRUCTURE_2ydz| PDB=2ydz | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ydz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ydz OCA], [https://pdbe.org/2ydz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ydz RCSB], [https://www.ebi.ac.uk/pdbsum/2ydz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ydz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cyan variants of green fluorescent protein are widely used as donors in Forster resonance energy transfer experiments. The popular, but modestly bright, Enhanced Cyan Fluorescent Protein (ECFP) was sequentially improved into the brighter variants Super Cyan Fluorescent Protein 3A (SCFP3A) and mTurquoise, the latter exhibiting a high-fluorescence quantum yield and a long mono-exponential fluorescence lifetime. Here we combine X-ray crystallography and excited-state calculations to rationalize these stepwise improvements. The enhancement originates from stabilization of the seventh beta-strand and the strengthening of the sole chromophore-stabilizing hydrogen bond. The structural analysis highlighted one suboptimal internal residue, which was subjected to saturation mutagenesis combined with fluorescence lifetime-based screening. This resulted in mTurquoise2, a brighter variant with faster maturation, high photostability, longer mono-exponential lifetime and the highest quantum yield measured for a monomeric fluorescent protein. Together, these properties make mTurquoise2 the preferable cyan variant of green fluorescent protein for long-term imaging and as donor for Forster resonance energy transfer to a yellow fluorescent protein.
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===X-ray structure of the cyan fluorescent protein SCFP3A (K206A mutant)===
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Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%.,Goedhart J, von Stetten D, Noirclerc-Savoye M, Lelimousin M, Joosen L, Hink MA, van Weeren L, Gadella TW Jr, Royant A Nat Commun. 2012 Mar 20;3:751. doi: 10.1038/ncomms1738. PMID:22434194<ref>PMID:22434194</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2ydz" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_22434194}}, adds the Publication Abstract to the page
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 22434194 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_22434194}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[2ydz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YDZ OCA].
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==Reference==
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<ref group="xtra">PMID:022434194</ref><ref group="xtra">PMID:016716067</ref><references group="xtra"/>
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[[Category: Aequorea victoria]]
[[Category: Aequorea victoria]]
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[[Category: Gadella, T W.J.]]
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[[Category: Large Structures]]
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[[Category: Goedhart, J.]]
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[[Category: Gadella TWJ]]
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[[Category: Hink, M A.]]
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[[Category: Goedhart J]]
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[[Category: Joosen, L.]]
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[[Category: Hink MA]]
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[[Category: Lelimousin, M.]]
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[[Category: Joosen L]]
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[[Category: Noirclerc-Savoye, M.]]
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[[Category: Lelimousin M]]
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[[Category: Royant, A.]]
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[[Category: Noirclerc-Savoye M]]
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[[Category: Stetten, D Von.]]
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[[Category: Royant A]]
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[[Category: Weeren, L Van.]]
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[[Category: Van Weeren L]]
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[[Category: Fluorescent protein]]
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[[Category: Von Stetten D]]
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[[Category: Fret donor]]
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Current revision

X-ray structure of the cyan fluorescent protein SCFP3A (K206A mutant)

PDB ID 2ydz

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