2wey

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[[Image:2wey.png|left|200px]]
 
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==Human PDE-papaverine complex obtained by ligand soaking of cross- linked protein crystals==
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The line below this paragraph, containing "STRUCTURE_2wey", creates the "Structure Box" on the page.
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<StructureSection load='2wey' size='340' side='right'caption='[[2wey]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2wey]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WEY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WEY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EV1:1-(3,4-DIMETHOXYBENZYL)-6,7-DIMETHOXYISOQUINOLINE'>EV1</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_2wey| PDB=2wey | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wey OCA], [https://pdbe.org/2wey PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wey RCSB], [https://www.ebi.ac.uk/pdbsum/2wey PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wey ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PDE10_HUMAN PDE10_HUMAN] Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate.<ref>PMID:17389385</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/we/2wey_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wey ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Protein crystallography has proven to be an effective method of obtaining high-resolution structures of protein-ligand complexes. However, in certain cases only apoprotein structures are readily available and the generation of crystal complexes is more problematic. Some crystallographic systems are not amenable to soaking of ligands owing to crystal-packing effects and many protein-ligand complexes do not crystallize under the same conditions as used for the apoprotein. Using crystals of human phosphodiesterase 10a (hPDE10a) as an example of such a challenging crystallographic system, the structure of the complex with papaverine was obtained to 2.8 A resolution using protein crystals cross-linked by glutaraldehyde prior to soaking of the ligand. Inspection of the electron-density maps suggested that the correct mode of binding was obtained in one of the two monomers in the asymmetric unit and inspection of crystal-packing contacts explained why cocrystallization experiments and soaking of crystals that were not cross-linked were unsuccessful.
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===Human PDE-papaverine complex obtained by ligand soaking of cross- linked protein crystals===
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Cross-linking of protein crystals as an aid in the generation of binary protein-ligand crystal complexes, exemplified by the human PDE10a-papaverine structure.,Andersen OA, Schonfeld DL, Toogood-Johnson I, Felicetti B, Albrecht C, Fryatt T, Whittaker M, Hallett D, Barker J Acta Crystallogr D Biol Crystallogr. 2009 Aug;65(Pt 8):872-4. Epub 2009, Jul 17. PMID:19622871<ref>PMID:19622871</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19622871}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2wey" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19622871 is the PubMed ID number.
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{{ABSTRACT_PUBMED_19622871}}
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==About this Structure==
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[[2wey]] is a 2 chain structure of [[Phosphodiesterase]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WEY OCA].
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==See Also==
==See Also==
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*[[Phosphodiesterase|Phosphodiesterase]]
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*[[Phosphodiesterase 3D structures|Phosphodiesterase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019622871</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Albrecht, C.]]
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[[Category: Large Structures]]
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[[Category: Andersen, O A.]]
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[[Category: Albrecht C]]
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[[Category: Barker, J.]]
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[[Category: Andersen OA]]
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[[Category: Felicetti, B.]]
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[[Category: Barker J]]
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[[Category: Fryatt, T.]]
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[[Category: Felicetti B]]
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[[Category: Hallett, D.]]
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[[Category: Fryatt T]]
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[[Category: Schonfeld, D L.]]
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[[Category: Hallett D]]
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[[Category: Toogood-Johnson, I.]]
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[[Category: Schonfeld DL]]
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[[Category: Whittaker, M.]]
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[[Category: Toogood-Johnson I]]
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[[Category: Allosteric enzyme]]
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[[Category: Whittaker M]]
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[[Category: Camp]]
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[[Category: Camp-binding]]
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[[Category: Cgmp]]
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[[Category: Cgmp-binding]]
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[[Category: Cross-linking]]
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[[Category: Hydrolase]]
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[[Category: Magnesium]]
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[[Category: Metal-binding]]
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[[Category: Nucleotide-binding]]
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[[Category: Papaverine]]
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[[Category: Phosphodiesterase]]
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[[Category: Phosphoprotein]]
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Current revision

Human PDE-papaverine complex obtained by ligand soaking of cross- linked protein crystals

PDB ID 2wey

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