4adv

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[[Image:4adv.png|left|200px]]
 
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==Structure of the E. coli methyltransferase KsgA bound to the E. coli 30S ribosomal subunit==
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The line below this paragraph, containing "STRUCTURE_4adv", creates the "Structure Box" on the page.
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<SX load='4adv' size='340' side='right' viewer='molstar' caption='[[4adv]], [[Resolution|resolution]] 13.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[4adv]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ADV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ADV FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 13.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4adv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4adv OCA], [https://pdbe.org/4adv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4adv RCSB], [https://www.ebi.ac.uk/pdbsum/4adv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4adv ProSAT]</span></td></tr>
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{{STRUCTURE_4adv| PDB=4adv | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RS2_ECOLI RS2_ECOLI]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The assembly of the ribosomal subunits is facilitated by ribosome biogenesis factors. The universally conserved methyltransferase KsgA modifies two adjacent adenosine residues in the 3'-terminal helix 45 of the 16 S ribosomal RNA (rRNA). KsgA recognizes its substrate adenosine residues only in the context of a near mature 30S subunit and is required for the efficient processing of the rRNA termini during ribosome biogenesis. Here, we present the cryo-EM structure of KsgA bound to a nonmethylated 30S ribosomal subunit. The structure reveals that KsgA binds to the 30S platform with the catalytic N-terminal domain interacting with substrate adenosine residues in helix 45 and the C-terminal domain making extensive contacts to helix 27 and helix 24. KsgA excludes the penultimate rRNA helix 44 from adopting its position in the mature 30S subunit, blocking the formation of the decoding site and subunit joining. We suggest that the activation of methyltransferase activity and subsequent dissociation of KsgA control conformational changes in helix 44 required for final rRNA processing and translation initiation.
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===Structure of the E. coli methyltransferase KsgA bound to the E. coli 30S ribosomal subunit===
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Structural Insights into Methyltransferase KsgA Function in 30S Ribosomal Subunit Biogenesis.,Boehringer D, O'Farrell HC, Rife JP, Ban N J Biol Chem. 2012 Mar 23;287(13):10453-9. Epub 2012 Feb 3. PMID:22308031<ref>PMID:22308031</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4adv" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_22308031}}, adds the Publication Abstract to the page
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*[[Adenosine dimethyltransferase 3D structures|Adenosine dimethyltransferase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 22308031 is the PubMed ID number.
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_22308031}}
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<references/>
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__TOC__
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==About this Structure==
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</SX>
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[[4adv]] is a 22 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ADV OCA].
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==Reference==
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<ref group="xtra">PMID:022308031</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Ban, N.]]
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[[Category: Large Structures]]
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[[Category: Boehringer, D.]]
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[[Category: Ban N]]
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[[Category: Farrell, H C.O.]]
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[[Category: Boehringer D]]
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[[Category: Rife, J P.]]
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[[Category: O'Farrell HC]]
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[[Category: Ribosome biogenesis]]
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[[Category: Rife JP]]
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[[Category: Small ribosomal subunit]]
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[[Category: Translation]]
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Current revision

Structure of the E. coli methyltransferase KsgA bound to the E. coli 30S ribosomal subunit

4adv, resolution 13.50Å

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