2ygy

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[[Image:2ygy.jpg|left|200px]]
 
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==Structure of wild type E. coli N-acetylneuraminic acid lyase in space group P21 crystal form II==
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The line below this paragraph, containing "STRUCTURE_2ygy", creates the "Structure Box" on the page.
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<StructureSection load='2ygy' size='340' side='right'caption='[[2ygy]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2ygy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_BL21(DE3) Escherichia coli BL21(DE3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YGY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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{{STRUCTURE_2ygy| PDB=2ygy | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ygy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ygy OCA], [https://pdbe.org/2ygy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ygy RCSB], [https://www.ebi.ac.uk/pdbsum/2ygy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ygy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NANA_ECOLI NANA_ECOLI] Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate.[HAMAP-Rule:MF_01237]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Twinning is a crystal growth anomaly, which has posed a challenge in macromolecular crystallography (MX) since the earliest days. Many approaches have been used to treat twinned data in order to extract structural information. However, in most cases it is usually simpler to rescreen for new crystallization conditions that yield an untwinned crystal form or, if possible, collect data from non-twinned parts of the crystal. Here, we report 11 structures of engineered variants of the E. coli enzyme N-acetyl-neuraminic lyase which, despite twinning and incommensurate modulation, have been successfully indexed, solved and deposited. These structures span a resolution range of 1.45-2.30 A, which is unusually high for datasets presenting such lattice disorders in MX and therefore these data provide an excellent test set for improving and challenging MX data processing programs.
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===Structure of wild type E. coli N-acetylneuraminic acid lyase in space group P21 crystal form II===
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Pathological macromolecular crystallographic data affected by twinning, partial-disorder and exhibiting multiple lattices for testing of data processing and refinement tools.,Campeotto I, Lebedev A, Schreurs AMM, Kroon-Batenburg LMJ, Lowe E, Phillips SEV, Murshudov GN, Pearson AR Sci Rep. 2018 Oct 5;8(1):14876. doi: 10.1038/s41598-018-32962-6. PMID:30291262<ref>PMID:30291262</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2ygy" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[2ygy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YGY OCA].
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*[[N-acetylneuraminate lyase 3D structures|N-acetylneuraminate lyase 3D structures]]
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[[Category: Escherichia coli]]
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== References ==
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[[Category: N-acetylneuraminate lyase]]
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<references/>
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[[Category: Berry, A.]]
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__TOC__
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[[Category: Campeotto, I.]]
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</StructureSection>
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[[Category: Nelson, A.]]
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[[Category: Large Structures]]
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[[Category: Pearson, A R.]]
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[[Category: Berry A]]
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[[Category: Phillips, S E.V.]]
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[[Category: Campeotto I]]
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[[Category: Directed evolution]]
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[[Category: Nelson A]]
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[[Category: Lyase]]
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[[Category: Pearson AR]]
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[[Category: Protein engineering]]
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[[Category: Phillips SEV]]
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[[Category: Substrate specificity]]
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Current revision

Structure of wild type E. coli N-acetylneuraminic acid lyase in space group P21 crystal form II

PDB ID 2ygy

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