This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1j8i

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:50, 3 April 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1j8i.jpg|left|200px]]<br /><applet load="1j8i" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1j8i" />
 
-
'''Solution Structure of Human Lymphotactin'''<br />
 
-
==Overview==
+
==Solution Structure of Human Lymphotactin==
-
Lymphotactin, the sole identified member of the C class of chemokines, specifically attracts T lymphocytes and natural killer cells. This, 93-residue protein lacks 2 of the 4 conserved cysteine residues, characteristic of the other 3 classes of chemokines and possesses an, extended carboxyl terminus, which is required for chemotactic activity. We, have determined the three-dimensional solution structure of recombinant, human lymphotactin by NMR spectroscopy. Under the conditions used for the, structure determination, lymphotactin was predominantly monomeric;, however, pulsed field gradient NMR self-diffusion measurements and, analytical ultracentrifugation revealed evidence of dimer formation., Sequence-specific chemical shift assignments were determined through, analysis of two- and three-dimensional NMR spectra of (15)N- and, (13)C/(15)N-enriched protein samples. Input for the torsion angle dynamics, calculations used in determining the structure included 1258 unique, NOE-derived distance constraints and 60 dihedral angle constraints, obtained from chemical-shift-based searching of a protein conformational, database. The ensemble of 20 structures chosen to represent the structure, had backbone and heavy atom rms deviations of 0.46 +/- 0.11 and 1.02 +/-, 0.14 A, respectively. The results revealed that human lymphotactin adopts, the conserved chemokine fold, which is characterized by a three-stranded, antiparallel beta-sheet and a C-terminal alpha-helix. Two regions are, dynamically disordered as evidenced by (1)H and (13)C chemical shifts and, [(15)N]-(1)H NOEs: residues 1-9 of the amino terminus and residues 69-93, of the C-terminal extension. A functional role for the C-terminal, extension, which is unique to lymphotactin, remains to be elucidated.
+
<StructureSection load='1j8i' size='340' side='right'caption='[[1j8i]]' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1j8i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J8I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J8I FirstGlance]. <br>
-
1J8I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J8I OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j8i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j8i OCA], [https://pdbe.org/1j8i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j8i RCSB], [https://www.ebi.ac.uk/pdbsum/1j8i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j8i ProSAT]</span></td></tr>
-
==Reference==
+
</table>
-
Monomeric solution structure of the prototypical 'C' chemokine lymphotactin., Kuloglu ES, McCaslin DR, Kitabwalla M, Pauza CD, Markley JL, Volkman BF, Biochemistry. 2001 Oct 23;40(42):12486-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11601972 11601972]
+
== Function ==
 +
[https://www.uniprot.org/uniprot/XCL1_HUMAN XCL1_HUMAN] Chemotactic activity for lymphocytes but not for monocytes or neutrophils.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j8/1j8i_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j8i ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Kuloglu, E.S.]]
+
[[Category: Kuloglu ES]]
-
[[Category: Markley, J.L.]]
+
[[Category: Markley JL]]
-
[[Category: McCaslin, D.R.]]
+
[[Category: McCaslin DR]]
-
[[Category: Pauza, C.D.]]
+
[[Category: Pauza CD]]
-
[[Category: Volkman, B.F.]]
+
[[Category: Volkman BF]]
-
[[Category: chemokine]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:06:07 2008''
+

Current revision

Solution Structure of Human Lymphotactin

PDB ID 1j8i

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1j8i"
Personal tools