1jhj

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[[Image:1jhj.jpg|left|200px]]<br /><applet load="1jhj" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jhj, resolution 1.6&Aring;" />
 
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'''Crystal structure of the APC10/Doc1 subunit of the human anaphase-promoting complex'''<br />
 
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==Overview==
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==Crystal structure of the APC10/Doc1 subunit of the human anaphase-promoting complex==
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The anaphase-promoting complex (APC), or cyclosome, is a cell, cycle-regulated ubiquitin ligase that controls progression through mitosis, and the G1 phase of the cell cycle. The APC is composed of at least 11, subunits; no structure has been determined for any of these subunits. The, subunit APC10/DOC1, a one-domain protein consisting of 185 amino acids, has a conserved core (residues 22-161) that is homologous to domains found, in several other putative ubiquitin ligases and, therefore, may play a, role in ubiquitination reactions. Here we report the crystal structure of, human APC10 at 1.6 A resolution. The core of the protein is formed by a, beta-sandwich that adopts a jellyroll fold. Unexpectedly, this structure, is highly similar to ligand-binding domains of several bacterial and, eukaryotic proteins, such as galactose oxidase and coagulation factor Va, raising the possibility that APC10 may function by binding a yet, unidentified ligand. We further provide biochemical evidence that the, C-terminus of APC10 binds to CDC27/APC3, an APC subunit that contains, multiple tetratrico peptide repeats.
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<StructureSection load='1jhj' size='340' side='right'caption='[[1jhj]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jhj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JHJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JHJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jhj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jhj OCA], [https://pdbe.org/1jhj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jhj RCSB], [https://www.ebi.ac.uk/pdbsum/1jhj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jhj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/APC10_HUMAN APC10_HUMAN] Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.<ref>PMID:18485873</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jh/1jhj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jhj ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The anaphase-promoting complex (APC), or cyclosome, is a cell cycle-regulated ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC is composed of at least 11 subunits; no structure has been determined for any of these subunits. The subunit APC10/DOC1, a one-domain protein consisting of 185 amino acids, has a conserved core (residues 22-161) that is homologous to domains found in several other putative ubiquitin ligases and, therefore, may play a role in ubiquitination reactions. Here we report the crystal structure of human APC10 at 1.6 A resolution. The core of the protein is formed by a beta-sandwich that adopts a jellyroll fold. Unexpectedly, this structure is highly similar to ligand-binding domains of several bacterial and eukaryotic proteins, such as galactose oxidase and coagulation factor Va, raising the possibility that APC10 may function by binding a yet unidentified ligand. We further provide biochemical evidence that the C-terminus of APC10 binds to CDC27/APC3, an APC subunit that contains multiple tetratrico peptide repeats.
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==About this Structure==
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Crystal structure of the APC10/DOC1 subunit of the human anaphase-promoting complex.,Wendt KS, Vodermaier HC, Jacob U, Gieffers C, Gmachl M, Peters JM, Huber R, Sondermann P Nat Struct Biol. 2001 Sep;8(9):784-8. PMID:11524682<ref>PMID:11524682</ref>
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1JHJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NI:'>NI</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JHJ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of the APC10/DOC1 subunit of the human anaphase-promoting complex., Wendt KS, Vodermaier HC, Jacob U, Gieffers C, Gmachl M, Peters JM, Huber R, Sondermann P, Nat Struct Biol. 2001 Sep;8(9):784-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11524682 11524682]
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</div>
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<div class="pdbe-citations 1jhj" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Gieffers, C.]]
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[[Category: Gieffers C]]
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[[Category: Gmachl, M.]]
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[[Category: Gmachl M]]
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[[Category: Huber, R.]]
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[[Category: Huber R]]
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[[Category: Jacob, U.]]
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[[Category: Jacob U]]
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[[Category: Peters, J.M.]]
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[[Category: Peters J-M]]
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[[Category: Sondermann, P.]]
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[[Category: Sondermann P]]
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[[Category: Vodermaier, H.C.]]
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[[Category: Vodermaier HC]]
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[[Category: Wendt, K.S.]]
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[[Category: Wendt KS]]
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[[Category: NI]]
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[[Category: beta sandwich]]
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[[Category: jellyroll]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:07:30 2008''
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Current revision

Crystal structure of the APC10/Doc1 subunit of the human anaphase-promoting complex

PDB ID 1jhj

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