1gw8

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[[Image:1gw8.png|left|200px]]
 
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==quasi-atomic resolution model of bacteriophage PRD1 sus607 mutant, obtained by combined cryo-EM and X-ray crystallography.==
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The line below this paragraph, containing "STRUCTURE_1gw8", creates the "Structure Box" on the page.
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<SX load='1gw8' size='340' side='right' viewer='molstar' caption='[[1gw8]], [[Resolution|resolution]] 13.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1gw8]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_PRD1 Enterobacteria phage PRD1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GW8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GW8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 13.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gw8 OCA], [https://pdbe.org/1gw8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gw8 RCSB], [https://www.ebi.ac.uk/pdbsum/1gw8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gw8 ProSAT]</span></td></tr>
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{{STRUCTURE_1gw8| PDB=1gw8 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CAPSD_BPPRD CAPSD_BPPRD] Major capsid protein self-assembles to form an icosahedral capsid with a pseudo T=25 symmetry, about 66 nm in diameter, and consisting of 240 capsid proteins trimers. The capsid encapsulates an inner membrane and the genomic dsDNA genome. The major coat protein P3 and two assembly factors (P10 and P17) are needed during the assembly of the virus particle inside the host cell, when the capsid protein multimers are capable of enclosing the host-derived membrane, containing the virus-encoded membrane-associated proteins.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacteriophage PRD1 shares many structural and functional similarities with adenovirus. A major difference is the PRD1 internal membrane, which acts in concert with vertex proteins to translocate the phage genome into the host. Multiresolution models of the PRD1 capsid, together with genetic analyses, provide fine details of the molecular interactions associated with particle stability and membrane dynamics. The N- and C-termini of the major coat protein (P3), which are required for capsid assembly, act as conformational switches bridging capsid to membrane and linking P3 trimers. Electrostatic P3-membrane interactions increase virion stability upon DNA packaging. Newly revealed proteins suggest how the metastable vertex works and how the capsid edges are stabilized.
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===quasi-atomic resolution model of bacteriophage PRD1 sus607 mutant, obtained by combined cryo-EM and X-ray crystallography.===
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Minor proteins, mobile arms and membrane-capsid interactions in the bacteriophage PRD1 capsid.,San Martin C, Huiskonen JT, Bamford JK, Butcher SJ, Fuller SD, Bamford DH, Burnett RM Nat Struct Biol. 2002 Oct;9(10):756-63. PMID:12219080<ref>PMID:12219080</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_12219080}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1gw8" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 12219080 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12219080}}
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__TOC__
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</SX>
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==About this Structure==
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[[Category: Enterobacteria phage PRD1]]
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[[1gw8]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_prd1 Enterobacteria phage prd1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GW8 OCA].
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[[Category: Large Structures]]
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[[Category: Bamford DH]]
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==Reference==
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[[Category: Bamford JKH]]
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<ref group="xtra">PMID:012219080</ref><ref group="xtra">PMID:011752778</ref><ref group="xtra">PMID:011591347</ref><ref group="xtra">PMID:010499799</ref><references group="xtra"/>
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[[Category: Burnett RM]]
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[[Category: Enterobacteria phage prd1]]
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[[Category: Butcher SJ]]
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[[Category: Bamford, D H.]]
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[[Category: Fuller SD]]
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[[Category: Bamford, J K.H.]]
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[[Category: Huiskonen J]]
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[[Category: Burnett, R M.]]
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[[Category: San Martin C]]
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[[Category: Butcher, S J.]]
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[[Category: Fuller, S D.]]
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[[Category: Huiskonen, J T.]]
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[[Category: Martin, C San.]]
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[[Category: Bacteriophage prd1]]
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[[Category: Cryo- em]]
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[[Category: Icosahedral virus]]
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[[Category: Image reconstruction]]
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[[Category: Tectiviridae]]
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[[Category: Virus-viral protein complex]]
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[[Category: Virus/viral protein]]
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Current revision

quasi-atomic resolution model of bacteriophage PRD1 sus607 mutant, obtained by combined cryo-EM and X-ray crystallography.

1gw8, resolution 13.30Å

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OCA

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