3egi

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[[Image:3egi.png|left|200px]]
 
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==Methyltransferase domain of human trimethylguanosine synthase TGS1 bound to m7GpppA (inactive form)==
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The line below this paragraph, containing "STRUCTURE_3egi", creates the "Structure Box" on the page.
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<StructureSection load='3egi' size='340' side='right'caption='[[3egi]], [[Resolution|resolution]] 2.21&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3egi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EGI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EGI FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.21&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3egi| PDB=3egi | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3egi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3egi OCA], [https://pdbe.org/3egi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3egi RCSB], [https://www.ebi.ac.uk/pdbsum/3egi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3egi ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TGS1_HUMAN TGS1_HUMAN] Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation.<ref>PMID:11517327</ref> <ref>PMID:11912212</ref> <ref>PMID:16687569</ref> <ref>PMID:18775984</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eg/3egi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3egi ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Methyltransferases play an important role in the post-transcriptional maturation of most ribonucleic acids. The modification of spliceosomal UsnRNAs includes N2-dimethylation of the m(7)G cap catalyzed by trimethylguanosine synthase 1 (TGS1). This 5'-cap hypermethylation occurs during the biogenesis of UsnRNPs as it initiates the m(3)G cap-dependent nuclear import of UsnRNPs. The conserved methyltransferase domain of human TGS1 has been purified, crystallized and the crystal structure of this domain with bound substrate m(7)GpppA was solved by means of multiple-wavelength anomalous dispersion. Crystal structure analysis revealed that m(7)GpppA binds via its adenosine moiety to the structurally conserved adenosylmethionine-binding pocket, while the m(7) guanosine remains unbound. This unexpected binding only occurs in the absence of AdoMet and suggests an incomplete binding pocket for the m(7)G cap which is caused by the N-terminal truncation of the protein. These structural data are consistent with the finding that the crystallized fragment of human TGS1 is catalytically inactive, while a fragment that is 17 amino acids longer exhibits activity.
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===Methyltransferase domain of human trimethylguanosine synthase TGS1 bound to m7GpppA (inactive form)===
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Structure analysis of the conserved methyltransferase domain of human trimethylguanosine synthase TGS1.,Monecke T, Dickmanns A, Strasser A, Ficner R Acta Crystallogr D Biol Crystallogr. 2009 Apr;65(Pt 4):332-8. Epub 2009 Mar 19. PMID:19307714<ref>PMID:19307714</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19307714}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3egi" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19307714 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19307714}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[3egi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EGI OCA].
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==Reference==
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<ref group="xtra">PMID:019307714</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Dickmanns, A.]]
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[[Category: Large Structures]]
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[[Category: Ficner, R.]]
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[[Category: Dickmanns A]]
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[[Category: Monecke, T.]]
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[[Category: Ficner R]]
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[[Category: Alpha-beta-alpha sandwich]]
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[[Category: Monecke T]]
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[[Category: Methyltransferase]]
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[[Category: Methyltransferase-domain]]
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[[Category: Nucleus]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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[[Category: Transferase]]
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Current revision

Methyltransferase domain of human trimethylguanosine synthase TGS1 bound to m7GpppA (inactive form)

PDB ID 3egi

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