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3ju4

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[[Image:3ju4.png|left|200px]]
 
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==Crystal Structure Analysis of EndosialidaseNF at 0.98 A Resolution==
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The line below this paragraph, containing "STRUCTURE_3ju4", creates the "Structure Box" on the page.
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<StructureSection load='3ju4' size='340' side='right'caption='[[3ju4]], [[Resolution|resolution]] 0.98&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ju4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_K1F Escherichia phage K1F]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JU4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JU4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.98&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SLB:5-N-ACETYL-BETA-D-NEURAMINIC+ACID'>SLB</scene></td></tr>
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{{STRUCTURE_3ju4| PDB=3ju4 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ju4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ju4 OCA], [https://pdbe.org/3ju4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ju4 RCSB], [https://www.ebi.ac.uk/pdbsum/3ju4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ju4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FIBER_BPK1F FIBER_BPK1F] Responsible for initial absorption of the phage to the host bacterium. Degrades the alpha-2,8-linked polysialic acid K1 capsule by cleaving within the polymer chain of polysialic acid.<ref>PMID:20096705</ref> <ref>PMID:3546309</ref> The C-terminal chaperone protein mediates homotrimerization and proper folding of the catalytic endo-N trimer.<ref>PMID:12556457</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ju/3ju4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ju4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Endosialidase NF (endoNF) is a bacteriophage-derived endosialidase that specifically degrades alpha-2,8-linked polysialic acid. The structure of a new crystal form of endoNF in complex with sialic acid has been refined at 0.98 A resolution. The 210 kDa homotrimeric multi-domain enzyme displays outstanding stability and resistance to SDS. Even at atomic resolution, only a minor fraction of side chains possess alternative conformations. However, multiple conformations of an active-site residue imply that it has an important catalytic function in the cleavage mechanism of polysialic acid.
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===Crystal Structure Analysis of EndosialidaseNF at 0.98 A Resolution===
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Structure analysis of endosialidase NF at 0.98 A resolution.,Schulz EC, Neumann P, Gerardy-Schahn R, Sheldrick GM, Ficner R Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):176-80. Epub 2010 Jan 22. PMID:20124697<ref>PMID:20124697</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20124697}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3ju4" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20124697 is the PubMed ID number.
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{{ABSTRACT_PUBMED_20124697}}
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==About this Structure==
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[[3ju4]] is a 1 chain structure of [[Neuraminidase]] with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_k1f Enterobacteria phage k1f]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JU4 OCA].
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==See Also==
==See Also==
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*[[Neuraminidase|Neuraminidase]]
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*[[Neuraminidase 3D structures|Neuraminidase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020124697</ref><references group="xtra"/>
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__TOC__
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[[Category: Endo-alpha-sialidase]]
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</StructureSection>
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[[Category: Enterobacteria phage k1f]]
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[[Category: Escherichia phage K1F]]
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[[Category: Ficner, R.]]
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[[Category: Large Structures]]
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[[Category: Gerardy-Schahn, R.]]
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[[Category: Ficner R]]
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[[Category: Neuman, P.]]
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[[Category: Gerardy-Schahn R]]
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[[Category: Schulz, E C.]]
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[[Category: Neuman P]]
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[[Category: Sheldrick, G M.]]
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[[Category: Schulz EC]]
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[[Category: 1a]]
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[[Category: Sheldrick GM]]
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[[Category: Endonf]]
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[[Category: Glycosidase]]
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[[Category: High-resolution]]
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[[Category: Hydrolase]]
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[[Category: Polysia]]
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Current revision

Crystal Structure Analysis of EndosialidaseNF at 0.98 A Resolution

PDB ID 3ju4

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