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1kl9

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[[Image:1kl9.jpg|left|200px]]<br /><applet load="1kl9" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1kl9, resolution 1.90&Aring;" />
 
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'''Crystal structure of the N-terminal segment of Human eukaryotic initiation factor 2alpha'''<br />
 
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==Overview==
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==Crystal structure of the N-terminal segment of Human eukaryotic initiation factor 2alpha==
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Eukaryotic translation initiation factor 2alpha (eIF2alpha) is a member of, the eIF2 heterotrimeric complex that binds and delivers Met-tRNA(i)(Met), to the 40 S ribosomal subunit in a GTP-dependent manner., Phosphorylation/dephosphorylation of eIF2alpha at Ser-51 is the major, regulator of protein synthesis in eukaryotic cells. Here, we report the, first structural analysis on eIF2, the three-dimensional structure of a, 22-kDa N-terminal portion of human eIF2alpha by x-ray diffraction at 1.9 A, resolution. This structure contains two major domains. The N terminus is a, beta-barrel with five antiparallel beta-strands in an oligonucleotide, binding domain (OB domain) fold. The phosphorylation site (Ser-51) is on, the loop connecting beta3 and beta4 in the OB domain. A helical domain, follows the OB domain, and the first helix has extensive interactions, including a disulfide bridge, to fix its orientation with respect to the, OB domain. The two domains meet along a negatively charged groove with, highly conserved residues, indicating a likely site for protein-protein, interaction.
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<StructureSection load='1kl9' size='340' side='right'caption='[[1kl9]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1kl9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KL9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KL9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kl9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kl9 OCA], [https://pdbe.org/1kl9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kl9 RCSB], [https://www.ebi.ac.uk/pdbsum/1kl9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kl9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IF2A_HUMAN IF2A_HUMAN] Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kl/1kl9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kl9 ConSurf].
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<div style="clear:both"></div>
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==Disease==
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==See Also==
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Known disease associated with this structure: Wolcott-Rallison syndrome OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=604032 604032]]
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*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
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__TOC__
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==About this Structure==
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</StructureSection>
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1KL9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KL9 OCA].
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==Reference==
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Crystal structure of the N-terminal segment of human eukaryotic translation initiation factor 2alpha., Nonato MC, Widom J, Clardy J, J Biol Chem. 2002 May 10;277(19):17057-61. Epub 2002 Feb 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11859078 11859078]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Clardy, J.]]
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[[Category: Clardy J]]
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[[Category: Nonato, M.C.]]
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[[Category: Nonato MC]]
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[[Category: Widom, J.]]
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[[Category: Widom J]]
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[[Category: ZN]]
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[[Category: helical domain]]
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[[Category: ob fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:14:26 2008''
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Current revision

Crystal structure of the N-terminal segment of Human eukaryotic initiation factor 2alpha

PDB ID 1kl9

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