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1l2h

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[[Image:1l2h.jpg|left|200px]]<br /><applet load="1l2h" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1l2h, resolution 1.54&Aring;" />
 
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'''Crystal structure of Interleukin 1-beta F42W/W120F mutant'''<br />
 
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==Overview==
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==Crystal structure of Interleukin 1-beta F42W/W120F mutant==
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The crystal structure at 1.54 A resolution of a double mutant of, interleukin-1beta (F42W/W120F), a cytokine secreted by macrophages, was, determined by multiple-wavelength anomalous dispersion (MAD) using data, from highly twinned selenomethionine-modified crystals. The space group is, P4(3), with unit-cell parameters a = b = 53.9, c = 77.4 A. Self-rotation, function analysis and various intensity statistics revealed the presence, of merohedral twinning in crystals of both the native (twinning fraction, alpha approximately 0.35) and SeMet (alpha approximately 0.40) forms., Structure determination and refinement are discussed with emphasis on the, possible reasons for successful phasing using untreated twinned MAD data.
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<StructureSection load='1l2h' size='340' side='right'caption='[[1l2h]], [[Resolution|resolution]] 1.54&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1l2h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L2H FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.54&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l2h OCA], [https://pdbe.org/1l2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l2h RCSB], [https://www.ebi.ac.uk/pdbsum/1l2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l2h ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IL1B_HUMAN IL1B_HUMAN] Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.<ref>PMID:3920526</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l2/1l2h_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l2h ConSurf].
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<div style="clear:both"></div>
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==Disease==
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==See Also==
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Known disease associated with this structure: Gastric cancer risk after H. pylori infection OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=147720 147720]]
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*[[Interleukin 3D structures|Interleukin 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1L2H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2H OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Use of multiple anomalous dispersion to phase highly merohedrally twinned crystals of interleukin-1beta., Rudolph MG, Kelker MS, Schneider TR, Yeates TO, Oseroff V, Heidary DK, Jennings PA, Wilson IA, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):290-8. Epub 2003, Jan 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12554939 12554939]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Heidary, D.K.]]
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[[Category: Heidary DK]]
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[[Category: Jennings, P.A.]]
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[[Category: Jennings PA]]
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[[Category: Kelker, M.S.]]
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[[Category: Kelker MS]]
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[[Category: Oseroff, V.]]
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[[Category: Oseroff V]]
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[[Category: Rudolph, M.G.]]
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[[Category: Rudolph MG]]
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[[Category: Schneider, T.R.]]
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[[Category: Schneider TR]]
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[[Category: Wilson, I.A.]]
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[[Category: Wilson IA]]
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[[Category: Yeates, T.O.]]
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[[Category: Yeates TO]]
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[[Category: beta-trefoil]]
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[[Category: f42w/w120f double mutant]]
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[[Category: hemihedral twinning]]
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[[Category: interleukin-1 beta]]
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[[Category: mad phasing]]
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[[Category: protein folding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:16:15 2008''
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Current revision

Crystal structure of Interleukin 1-beta F42W/W120F mutant

PDB ID 1l2h

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