1lqs

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[[Image:1lqs.jpg|left|200px]]<br /><applet load="1lqs" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1lqs, resolution 2.7&Aring;" />
 
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'''CRYSTAL STRUCTURE OF HUMAN CYTOMEGALOVIRUS IL-10 BOUND TO SOLUBLE HUMAN IL-10R1'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF HUMAN CYTOMEGALOVIRUS IL-10 BOUND TO SOLUBLE HUMAN IL-10R1==
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Human IL-10 (hIL-10) modulates critical immune and inflammatory responses, by way of interactions with its high- (IL-10R1) and low-affinity (IL-10R2), cell surface receptors. Human cytomegalovirus exploits the IL-10 signaling, pathway by expressing a functional viral IL-10 homolog (cmvIL-10), which, shares only 27% sequence identity with hIL-10 yet signals through IL-10R1, and IL-10R2. To define the molecular basis of this virus-host interaction, we determined the 2.7-A crystal structure of cmvIL-10 bound to the, extracellular fragment of IL-10R1 (sIL-10R1). The structure reveals, cmvIL-10 forms a disulfide-linked homodimer that binds two sIL-10R1, molecules. Although cmvIL-10 and hIL-10 share similar intertwined, topologies and sIL-10R1 binding sites, their respective interdomain angles, differ by approximately 40 degrees. This difference results in a striking, re-organization of the IL-10R1s in the putative cell surface complex., Solution binding studies show cmvIL-10 and hIL-10 share essentially, identical affinities for sIL-10R1 whereas the Epstein-Barr virus IL-10, homolog (ebvIL-10), whose structure is highly similar to hIL-10, exhibits, a approximately 20-fold reduction in sIL-10R1 affinity. Our results, suggest cmvIL-10 and ebvIL-10 have evolved different molecular mechanisms, to engage the IL-10 receptors that ultimately enhance the respective, ability of their virus to escape immune detection.
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<StructureSection load='1lqs' size='340' side='right'caption='[[1lqs]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1lqs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Human_betaherpesvirus_5 Human betaherpesvirus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LQS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LQS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lqs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lqs OCA], [https://pdbe.org/1lqs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lqs RCSB], [https://www.ebi.ac.uk/pdbsum/1lqs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lqs ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/I10R1_HUMAN I10R1_HUMAN] Defects in IL10RA are the cause of inflammatory bowel disease type 28 (IBD28) [MIM:[https://omim.org/entry/613148 613148]. It is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.<ref>PMID:19890111</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/I10R1_HUMAN I10R1_HUMAN] Receptor for IL10; binds IL10 with a high affinity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lq/1lqs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lqs ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Human IL-10 (hIL-10) modulates critical immune and inflammatory responses by way of interactions with its high- (IL-10R1) and low-affinity (IL-10R2) cell surface receptors. Human cytomegalovirus exploits the IL-10 signaling pathway by expressing a functional viral IL-10 homolog (cmvIL-10), which shares only 27% sequence identity with hIL-10 yet signals through IL-10R1 and IL-10R2. To define the molecular basis of this virus-host interaction, we determined the 2.7-A crystal structure of cmvIL-10 bound to the extracellular fragment of IL-10R1 (sIL-10R1). The structure reveals cmvIL-10 forms a disulfide-linked homodimer that binds two sIL-10R1 molecules. Although cmvIL-10 and hIL-10 share similar intertwined topologies and sIL-10R1 binding sites, their respective interdomain angles differ by approximately 40 degrees. This difference results in a striking re-organization of the IL-10R1s in the putative cell surface complex. Solution binding studies show cmvIL-10 and hIL-10 share essentially identical affinities for sIL-10R1 whereas the Epstein-Barr virus IL-10 homolog (ebvIL-10), whose structure is highly similar to hIL-10, exhibits a approximately 20-fold reduction in sIL-10R1 affinity. Our results suggest cmvIL-10 and ebvIL-10 have evolved different molecular mechanisms to engage the IL-10 receptors that ultimately enhance the respective ability of their virus to escape immune detection.
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==About this Structure==
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Crystal structure of human cytomegalovirus IL-10 bound to soluble human IL-10R1.,Jones BC, Logsdon NJ, Josephson K, Cook J, Barry PA, Walter MR Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9404-9. Epub 2002 Jul 1. PMID:12093920<ref>PMID:12093920</ref>
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1LQS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Human_herpesvirus_5 Human herpesvirus 5] with <scene name='pdbligand=NAG:'>NAG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LQS OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of human cytomegalovirus IL-10 bound to soluble human IL-10R1., Jones BC, Logsdon NJ, Josephson K, Cook J, Barry PA, Walter MR, Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9404-9. Epub 2002 Jul 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12093920 12093920]
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</div>
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[[Category: Homo sapiens]]
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<div class="pdbe-citations 1lqs" style="background-color:#fffaf0;"></div>
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[[Category: Human herpesvirus 5]]
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[[Category: Protein complex]]
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[[Category: Barry, P.A.]]
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[[Category: Cook, J.]]
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[[Category: Jones, B.C.]]
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[[Category: Josephson, K.]]
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[[Category: Logsdon, N.J.]]
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[[Category: Walter, M.R.]]
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[[Category: NAG]]
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[[Category: helix bundle]]
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[[Category: interleukin 10]]
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[[Category: molecular recognition]]
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[[Category: receptor complex]]
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[[Category: structure mimic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:19:57 2008''
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==See Also==
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*[[Interleukin 3D structures|Interleukin 3D structures]]
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*[[Interleukin receptor 3D structures|Interleukin receptor 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Human betaherpesvirus 5]]
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[[Category: Large Structures]]
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[[Category: Barry PA]]
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[[Category: Cook J]]
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[[Category: Jones BC]]
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[[Category: Josephson K]]
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[[Category: Logsdon NJ]]
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[[Category: Walter MR]]

Current revision

CRYSTAL STRUCTURE OF HUMAN CYTOMEGALOVIRUS IL-10 BOUND TO SOLUBLE HUMAN IL-10R1

PDB ID 1lqs

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