4dgj

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[[Image:4dgj.png|left|200px]]
 
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==Structure of a human enteropeptidase light chain variant==
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The line below this paragraph, containing "STRUCTURE_4dgj", creates the "Structure Box" on the page.
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<StructureSection load='4dgj' size='340' side='right'caption='[[4dgj]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[4dgj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DGJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DGJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dgj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dgj OCA], [https://pdbe.org/4dgj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dgj RCSB], [https://www.ebi.ac.uk/pdbsum/4dgj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dgj ProSAT]</span></td></tr>
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{{STRUCTURE_4dgj| PDB=4dgj | SCENE= }}
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/ENTK_HUMAN ENTK_HUMAN] Congenital enteropathy due to enteropeptidase deficiency. The disease is caused by mutations affecting the gene represented in this entry.
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== Function ==
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[https://www.uniprot.org/uniprot/ENTK_HUMAN ENTK_HUMAN] Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The highly specific serine protease human enteropeptidase light chain (hEPl) cleaves the Asp(4) Lys recognition sequence and represents an interesting enzyme for biotechnological applications. The human enzyme shows 10-times faster kinetics compared to other animal sources but low solubility under low salt conditions, which hampers protein production and crystallization. Therefore, a supercharged variant (N6D/G21D/G22D/N142D/K210E/C112S) with increased solubility was used for crystallization. The structure (1.9A resolution) displays a typical alpha/beta trypsin-like serine protease-fold. The mutations introduced for protein supercharging generate larger clusters of negative potential on both sites of the active cleft but do not affect the structural integrity of the protein. Proteins 2012. (c) 2012 Wiley-Liss, Inc.
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===Structure of a human enteropeptidase light chain variant===
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Crystal structure of a supercharged variant of the human enteropeptidase light chain.,Simeonov P, Zahn M, Strater N, Zuchner T Proteins. 2012 Apr 10. doi: 10.1002/prot.24084. PMID:22488687<ref>PMID:22488687</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_22488687}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 4dgj" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 22488687 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_22488687}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[4dgj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DGJ OCA].
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==Reference==
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<ref group="xtra">PMID:022488687</ref><references group="xtra"/>
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[[Category: Enteropeptidase]]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Simeonov, P.]]
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[[Category: Large Structures]]
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[[Category: Straeter, N.]]
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[[Category: Simeonov P]]
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[[Category: Zahn, M.]]
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[[Category: Straeter N]]
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[[Category: Hydrolase]]
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[[Category: Zahn M]]
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[[Category: Serine protease]]
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Current revision

Structure of a human enteropeptidase light chain variant

PDB ID 4dgj

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