2wsm

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[[Image:2wsm.png|left|200px]]
 
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==Crystal structure of Hydrogenase Maturation Factor HypB From Archaeoglobus Fulgidus==
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The line below this paragraph, containing "STRUCTURE_2wsm", creates the "Structure Box" on the page.
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<StructureSection load='2wsm' size='340' side='right'caption='[[2wsm]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2wsm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WSM FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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{{STRUCTURE_2wsm| PDB=2wsm | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wsm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wsm OCA], [https://pdbe.org/2wsm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wsm RCSB], [https://www.ebi.ac.uk/pdbsum/2wsm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wsm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O28903_ARCFU O28903_ARCFU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ws/2wsm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wsm ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Maturation of [NiFe]-hydrogenase requires the insertion of iron, cyanide and carbon monoxide, followed by nickel, to the catalytic core of the enzyme. Hydrogenase maturation factor HypB is a metal-binding GTPase that is essential for the nickel delivery to the hydrogenase. Here we report the crystal structure of Archeoglobus fulgidus HypB (AfHypB) in apo-form. We showed that AfHypB recognizes guanine nucleotide using Asp-194 on the G5 loop despite having a non-canonical NKxA G4-motif. Structural comparison with the GTPgammaS-bound Methanocaldococcus jannaschii HypB identifies conformational changes in the switch I region, which bring an invariant Asp-72 to form an intermolecular salt-bridge with another invariant residue Lys-148 upon GTP binding. Substitution of K148A abolished GTP-dependent dimerization of AfHypB, but had no significant effect on the guanine nucleotide binding and on the intrinsic GTPase activity. In vivo complementation study in Escherichia coli showed that the invariant lysine residue is required for in vivo maturation of hydrogenase. Taken together, our results suggest that GTP-dependent dimerization of HypB is essential for hydrogenase maturation. It is likely that a nickel ion is loaded to an extra metal binding site at the dimeric interface of GTP-bound HypB and transferred to the hydrogenase upon GTP hydrolysis.
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===Crystal structure of Hydrogenase Maturation Factor HypB From Archaeoglobus Fulgidus===
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Structural basis for GTP-dependent dimerization of hydrogenase maturation factor HypB.,Chan KH, Li T, Wong CO, Wong KB PLoS One. 2012;7(1):e30547. Epub 2012 Jan 20. PMID:22276211<ref>PMID:22276211</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2wsm" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_22276211}}, adds the Publication Abstract to the page
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*[[HypA%2C HypB%2C HypC%2C HypD%2C HypE and HypF 3D structures|HypA%2C HypB%2C HypC%2C HypD%2C HypE and HypF 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 22276211 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_22276211}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Archaeoglobus fulgidus DSM 4304]]
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[[2wsm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WSM OCA].
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[[Category: Large Structures]]
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[[Category: Li T]]
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==Reference==
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[[Category: Wong KB]]
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<ref group="xtra">PMID:022276211</ref><references group="xtra"/>
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[[Category: Archaeoglobus fulgidus]]
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[[Category: Li, T.]]
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[[Category: Wong, K B.]]
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[[Category: Metal binding protein]]
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Current revision

Crystal structure of Hydrogenase Maturation Factor HypB From Archaeoglobus Fulgidus

PDB ID 2wsm

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