3o77

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[[Image:3o77.png|left|200px]]
 
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==The structure of Ca2+ Sensor (Case-16)==
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The line below this paragraph, containing "STRUCTURE_3o77", creates the "Structure Box" on the page.
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<StructureSection load='3o77' size='340' side='right'caption='[[3o77]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3o77]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria], [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O77 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3o77| PDB=3o77 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o77 OCA], [https://pdbe.org/3o77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o77 RCSB], [https://www.ebi.ac.uk/pdbsum/3o77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o77 ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/CALM1_HUMAN CALM1_HUMAN] The disease is caused by mutations affecting the gene represented in this entry. Mutations in CALM1 are the cause of CPVT4. The disease is caused by mutations affecting the gene represented in this entry. Mutations in CALM1 are the cause of LQT14.
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== Function ==
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[https://www.uniprot.org/uniprot/CALM1_HUMAN CALM1_HUMAN] Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (PubMed:16760425). Mediates calcium-dependent inactivation of CACNA1C (PubMed:26969752). Positively regulates calcium-activated potassium channel activity of KCNN2 (PubMed:27165696).<ref>PMID:16760425</ref> <ref>PMID:23893133</ref> <ref>PMID:26969752</ref> <ref>PMID:27165696</ref> [https://www.uniprot.org/uniprot/MYLK_CHICK MYLK_CHICK] Phosphorylates a specific serine in the N-terminus of a myosin light chain, which leads to the formation of calmodulin/MLCK signal transduction complexes which allow selective transduction of calcium signals.[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Here we report the first crystal structure of a high-contrast genetically encoded circularly permuted green fluorescent protein (cpGFP)-based Ca(2+) sensor, Case16, in the presence of a low Ca(2+) concentration. The structure reveals the positioning of the chromophore within Case16 at the first stage of the Ca(2+)-dependent response when only two out of four Ca(2+)-binding pockets of calmodulin (CaM) are occupied with Ca(2+) ions. In such a "half Ca(2+)-bound state", Case16 is characterized by an incomplete interaction between its CaM-/M13-domains. We also report the crystal structure of the related Ca(2+) sensor Case12 at saturating Ca(2+) concentration. Based on this structure, we postulate that cpGFP-based Ca(2+) sensors can form non-functional homodimers where the CaM-domain of one sensor molecule binds symmetrically to the M13-peptide of the partner sensor molecule. Case12 and Case16 behavior upon addition of high concentrations of free CaM or M13-peptide reveals that the latter effectively blocks the fluorescent response of the sensor. We speculate that the demonstrated intermolecular interaction with endogenous substrates and homodimerization can impede proper functioning of this type of Ca(2+) sensors in living cells.
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===The structure of Ca2+ Sensor (Case-16)===
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The structure of Ca2+ sensor Case16 reveals the mechanism of reaction to low Ca2+ concentrations.,Leder L, Stark W, Freuler F, Marsh M, Meyerhofer M, Stettler T, Mayr LM, Britanova OV, Strukova LA, Chudakov DM, Souslova EA Sensors (Basel). 2010;10(9):8143-60. Epub 2010 Aug 30. PMID:22163646<ref>PMID:22163646</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3o77" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 22163646 is the PubMed ID number.
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{{ABSTRACT_PUBMED_22163646}}
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==About this Structure==
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[[3o77]] is a 1 chain structure of [[Alyssa Marsico/Sandbox 1]], [[Calmodulin]], [[Devon McCarthy/Sandbox 1]], [[Green Fluorescent Protein]], [[Sandbox104]] and [[User:Joanne Lau/Sandbox 5]] with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus,_aequorea_victoria,_aequorea_victoria,_homo_sapiens Gallus gallus, aequorea victoria, aequorea victoria, homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O77 OCA].
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==See Also==
==See Also==
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*[[Alyssa Marsico/Sandbox 1|Alyssa Marsico/Sandbox 1]]
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*[[Calmodulin 3D structures|Calmodulin 3D structures]]
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*[[Calmodulin|Calmodulin]]
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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*[[Devon McCarthy/Sandbox 1|Devon McCarthy/Sandbox 1]]
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== References ==
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*[[Green Fluorescent Protein|Green Fluorescent Protein]]
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<references/>
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*[[Sandbox104|Sandbox104]]
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__TOC__
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*[[User:Joanne Lau/Sandbox 5|User:Joanne Lau/Sandbox 5]]
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</StructureSection>
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[[Category: Aequorea victoria]]
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==Reference==
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[[Category: Gallus gallus]]
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<ref group="xtra">PMID:022163646</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
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[[Category: Gallus gallus, aequorea victoria, aequorea victoria, homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Britanova, O V.]]
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[[Category: Britanova OV]]
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[[Category: Chudakov, D M.]]
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[[Category: Chudakov DM]]
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[[Category: Freuler, F.]]
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[[Category: Freuler F]]
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[[Category: Leder, L.]]
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[[Category: Leder L]]
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[[Category: Marsh, M.]]
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[[Category: Marsh M]]
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[[Category: Mayr, L M.]]
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[[Category: Mayr LM]]
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[[Category: Meyerhofer, M.]]
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[[Category: Meyerhofer M]]
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[[Category: Stark, W.]]
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[[Category: Stark W]]
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[[Category: Stettler, T.]]
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[[Category: Stettler T]]
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[[Category: Strukova, L A.]]
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[[Category: Strukova LA]]
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[[Category: Calcium sensor]]
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[[Category: Circular permutated green fluorescent protein]]
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[[Category: Fluorescent calcium indicator protein]]
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[[Category: Fluorescent protein]]
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[[Category: Genetically encoded]]
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[[Category: Gfp calmodulin m13-peptide]]
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[[Category: Gyg naturally modified tripeptide acts as chromophore]]
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[[Category: M13-peptide]]
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Current revision

The structure of Ca2+ Sensor (Case-16)

PDB ID 3o77

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