4apv

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[[Image:4apv.jpg|left|200px]]
 
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==The Klebsiella pneumoniae primosomal PriB protein: identification, crystal structure, and ssDNA binding mode==
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The line below this paragraph, containing "STRUCTURE_4apv", creates the "Structure Box" on the page.
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<StructureSection load='4apv' size='340' side='right'caption='[[4apv]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[4apv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4APV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4APV FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.095&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4apv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4apv OCA], [https://pdbe.org/4apv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4apv RCSB], [https://www.ebi.ac.uk/pdbsum/4apv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4apv ProSAT]</span></td></tr>
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{{STRUCTURE_4apv| PDB=4apv | SCENE= }}
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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PriB is a primosomal DNA replication protein required for the re-initiation of replication in bacteria. In this study, we investigated the gene expression of PriB in Klebsiella pneumoniae (KpPriB) and characterized the gene product through crystal structural and functional analyses. Quantitative polymerase chain reaction analysis (Q-PCR) indicated that the 104-aa priB was expressed in K. pneumoniae with a C(T) value of 22.4. The crystal structure of KpPriB (Protein Data Bank entry: 4APV) determined at a resolution of 2.1 A was similar to that of Escherichia coli PriB (EcPriB). KpPriB formed a single complex with single-stranded DNA (ssDNA) of different lengths, suggesting a highly cooperative process. Structure-based mutational analysis revealed that substitution at K18, F42, R44, W47, K82, K84, or K89 but not R34 in KpPriB had a significant effect on both ssDNA and double-stranded DNA (dsDNA) binding. Based on these findings, the known ssDNA interaction sites of PriB were expanded to include R44 and F42, thus allowing nucleic acids to wrap around the whole PriB protein.
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===The Klebsiella pneumoniae primosomal PriB protein: identification, crystal structure, and ssDNA binding mode===
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Crystal structure and DNA-binding mode of Klebsiella pneumoniae primosomal PriB protein.,Huang YH, Lo YH, Huang W, Huang CY Genes Cells. 2012 Oct;17(10):837-49. doi: 10.1111/gtc.12001. Epub 2012 Sep 3. PMID:22938024<ref>PMID:22938024</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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[[4apv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4APV OCA].
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<div class="pdbe-citations 4apv" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Klebsiella pneumoniae]]
[[Category: Klebsiella pneumoniae]]
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[[Category: Hsiao, C D.]]
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[[Category: Large Structures]]
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[[Category: Huang, C Y.]]
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[[Category: Hsiao CD]]
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[[Category: Huang, Y H.]]
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[[Category: Huang CY]]
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[[Category: Lo, Y H.]]
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[[Category: Huang YH]]
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[[Category: Prib primosome ssdna binding]]
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[[Category: Lo YH]]
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[[Category: Replication]]
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Current revision

The Klebsiella pneumoniae primosomal PriB protein: identification, crystal structure, and ssDNA binding mode

PDB ID 4apv

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