This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1o7z

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (12:30, 13 December 2023) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1o7z.jpg|left|200px]]<br /><applet load="1o7z" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1o7z, resolution 1.92&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF IP-10 T-FORM'''<br />
 
-
==Overview==
+
==Crystal structure of IP-10 T-form==
-
We have determined the structure of wild-type IP-10 from three crystal, forms. The crystals provide eight separate models of the IP-10 chain, all, differing substantially from a monomeric IP-10 variant examined previously, by NMR spectroscopy. In each crystal form, IP-10 chains form conventional, beta sheet dimers, which, in turn, form a distinct tetrameric assembly., The M form tetramer is reminiscent of platelet factor 4, whereas the T and, H forms feature a novel twelve-stranded beta sheet. Analytical, ultracentrifugation indicates that, in free solution, IP-10 exists in a, monomer-dimer equilibrium with a dissociation constant of 9 microM. We, propose that the tetrameric structures may represent species promoted by, the binding of glycosaminoglycans. The binding sites for several, IP-10-neutralizing mAbs have also been mapped.
+
<StructureSection load='1o7z' size='340' side='right'caption='[[1o7z]], [[Resolution|resolution]] 1.92&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1o7z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O7Z FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.92&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7z OCA], [https://pdbe.org/1o7z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o7z RCSB], [https://www.ebi.ac.uk/pdbsum/1o7z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7z ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CXL10_HUMAN CXL10_HUMAN] Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/1o7z_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o7z ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
We have determined the structure of wild-type IP-10 from three crystal forms. The crystals provide eight separate models of the IP-10 chain, all differing substantially from a monomeric IP-10 variant examined previously by NMR spectroscopy. In each crystal form, IP-10 chains form conventional beta sheet dimers, which, in turn, form a distinct tetrameric assembly. The M form tetramer is reminiscent of platelet factor 4, whereas the T and H forms feature a novel twelve-stranded beta sheet. Analytical ultracentrifugation indicates that, in free solution, IP-10 exists in a monomer-dimer equilibrium with a dissociation constant of 9 microM. We propose that the tetrameric structures may represent species promoted by the binding of glycosaminoglycans. The binding sites for several IP-10-neutralizing mAbs have also been mapped.
-
==About this Structure==
+
Crystal structures of oligomeric forms of the IP-10/CXCL10 chemokine.,Swaminathan GJ, Holloway DE, Colvin RA, Campanella GK, Papageorgiou AC, Luster AD, Acharya KR Structure. 2003 May;11(5):521-32. PMID:12737818<ref>PMID:12737818</ref>
-
1O7Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7Z OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Crystal structures of oligomeric forms of the IP-10/CXCL10 chemokine., Swaminathan GJ, Holloway DE, Colvin RA, Campanella GK, Papageorgiou AC, Luster AD, Acharya KR, Structure. 2003 May;11(5):521-32. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12737818 12737818]
+
</div>
-
[[Category: Single protein]]
+
<div class="pdbe-citations 1o7z" style="background-color:#fffaf0;"></div>
-
[[Category: Acharya, K.R.]]
+
-
[[Category: Holloway, D.E.]]
+
-
[[Category: Papageorgiou, A.C.]]
+
-
[[Category: Swaminathan, G.J.]]
+
-
[[Category: chemokine]]
+
-
[[Category: chemotaxis]]
+
-
[[Category: inflammatory response]]
+
-
[[Category: interferon induction]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:33:19 2008''
+
==See Also==
 +
*[[C-X-C motif chemokine 3D structures|C-X-C motif chemokine 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Homo sapiens]]
 +
[[Category: Large Structures]]
 +
[[Category: Acharya KR]]
 +
[[Category: Holloway DE]]
 +
[[Category: Papageorgiou AC]]
 +
[[Category: Swaminathan GJ]]

Current revision

Crystal structure of IP-10 T-form

PDB ID 1o7z

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1o7z"
Personal tools