3i0n

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[[Image:3i0n.png|left|200px]]
 
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==Structure of the S. pombe Nbs1 FHA/BRCT-repeat domain==
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The line below this paragraph, containing "STRUCTURE_3i0n", creates the "Structure Box" on the page.
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<StructureSection load='3i0n' size='340' side='right'caption='[[3i0n]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3i0n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I0N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I0N FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene></td></tr>
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{{STRUCTURE_3i0n| PDB=3i0n | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i0n OCA], [https://pdbe.org/3i0n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i0n RCSB], [https://www.ebi.ac.uk/pdbsum/3i0n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i0n ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NBS1_SCHPO NBS1_SCHPO] Required for DNA damage repair and S-phase DNA damage checkpoint. Involved in telomere length maintenance and maintenance of chromatin structure.<ref>PMID:12944481</ref> <ref>PMID:12944482</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i0/3i0n_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i0n ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Mre11/Rad50/Nbs1 protein complex plays central enzymatic and signaling roles in the DNA-damage response. Nuclease (Mre11) and scaffolding (Rad50) components of MRN have been extensively characterized, but the molecular basis of Nbs1 function has remained elusive. Here, we present a 2.3A crystal structure of the N-terminal region of fission yeast Nbs1, revealing an unusual but conserved architecture in which the FHA- and BRCT-repeat domains structurally coalesce. We demonstrate that diphosphorylated pSer-Asp-pThr-Asp motifs, recently identified as multicopy docking sites within Mdc1, are evolutionarily conserved Nbs1 binding targets. Furthermore, we show that similar phosphomotifs within Ctp1, the fission yeast ortholog of human CtIP, promote interactions with the Nbs1 FHA domain that are necessary for Ctp1-dependent resistance to DNA damage. Finally, we establish that human Nbs1 interactions with Mdc1 occur through both its FHA- and BRCT-repeat domains, suggesting how their structural and functional interdependence underpins Nbs1 adaptor functions in the DNA-damage response.
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===Structure of the S. pombe Nbs1 FHA/BRCT-repeat domain===
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A supramodular FHA/BRCT-repeat architecture mediates Nbs1 adaptor function in response to DNA damage.,Lloyd J, Chapman JR, Clapperton JA, Haire LF, Hartsuiker E, Li J, Carr AM, Jackson SP, Smerdon SJ Cell. 2009 Oct 2;139(1):100-11. PMID:19804756<ref>PMID:19804756</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19804756}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3i0n" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19804756 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19804756}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[3i0n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I0N OCA].
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==Reference==
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<ref group="xtra">PMID:019804756</ref><references group="xtra"/>
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[[Category: Schizosaccharomyces pombe]]
[[Category: Schizosaccharomyces pombe]]
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[[Category: Chapman, J R.]]
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[[Category: Chapman JR]]
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[[Category: Clapperton, J A.]]
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[[Category: Clapperton JA]]
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[[Category: Jackson, S P.]]
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[[Category: Jackson SP]]
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[[Category: Lloyd, J.]]
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[[Category: Lloyd J]]
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[[Category: Smerdon, S J.]]
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[[Category: Smerdon SJ]]
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[[Category: Brct-repeat]]
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[[Category: Cell cycle]]
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[[Category: Chromosomal protein]]
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[[Category: Dna damage]]
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[[Category: Dna repair]]
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[[Category: Dna-damage]]
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[[Category: Fha]]
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[[Category: Gene regulation]]
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[[Category: Nbs1]]
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[[Category: Nucleus]]
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[[Category: Phosphoprotein]]
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[[Category: Telomere]]
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Current revision

Structure of the S. pombe Nbs1 FHA/BRCT-repeat domain

PDB ID 3i0n

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