3kch

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[[Image:3kch.png|left|200px]]
 
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==Baranase crosslinked by glutaraldehyde==
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The line below this paragraph, containing "STRUCTURE_3kch", creates the "Structure Box" on the page.
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<StructureSection load='3kch' size='340' side='right'caption='[[3kch]], [[Resolution|resolution]] 1.94&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3kch]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KCH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KCH FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PTD:PENTANEDIAL'>PTD</scene></td></tr>
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{{STRUCTURE_3kch| PDB=3kch | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kch OCA], [https://pdbe.org/3kch PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kch RCSB], [https://www.ebi.ac.uk/pdbsum/3kch PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kch ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNBR_BACAM RNBR_BACAM] Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kc/3kch_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kch ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In addition to the common use of glutaraldehyde to nonspecifically cross-link protein crystals through lysine residues disposed on the surface of the protein, the use of gentle vapour diffusion of glutaraldehyde offers a convenient way to limit polymerization and to allow slow diffusion throughout the crystal. In the case of trimeric barnase crystals, a specific cross-link was observed between an lysine side chain and an arginine side chain that were spatially disposed at the ideal distance on the protein surface in the three monomers. Here, the direct observation of a specific Lys-Arg cross-link site is reported and a mechanism is proposed for the reaction.
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===Baranase crosslinked by glutaraldehyde===
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Revisiting glutaraldehyde cross-linking: the case of the Arg-Lys intermolecular doublet.,Salem M, Mauguen Y, Prange T Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Mar 1;66(Pt, 3):225-8. Epub 2010 Feb 23. PMID:20208148<ref>PMID:20208148</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20208148}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3kch" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20208148 is the PubMed ID number.
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{{ABSTRACT_PUBMED_20208148}}
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==About this Structure==
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[[3kch]] is a 3 chain structure of [[Barnase]] and [[Ribonuclease]] with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KCH OCA].
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==See Also==
==See Also==
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*[[Barnase|Barnase]]
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*[[Barnase 3D structures|Barnase 3D structures]]
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*[[Ribonuclease|Ribonuclease]]
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020208148</ref><ref group="xtra">PMID:016600702</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Bacillus amyloliquefaciens]]
[[Category: Bacillus amyloliquefaciens]]
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[[Category: Mauguen, Y.]]
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[[Category: Large Structures]]
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[[Category: Prange, T.]]
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[[Category: Mauguen Y]]
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[[Category: Salem, M.]]
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[[Category: Prange T]]
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[[Category: Crosslink]]
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[[Category: Salem M]]
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[[Category: Endonuclease]]
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[[Category: Glutaraldehyde]]
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[[Category: Hydrolase]]
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[[Category: Microbial ribonuclease]]
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[[Category: Nuclease]]
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[[Category: Ribonuclease]]
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[[Category: Secreted]]
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Current revision

Baranase crosslinked by glutaraldehyde

PDB ID 3kch

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