This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1p4u

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:05, 14 February 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1p4u.jpg|left|200px]]<br /><applet load="1p4u" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1p4u, resolution 2.20&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF GGA3 GAE DOMAIN IN COMPLEX WITH RABAPTIN-5 PEPTIDE'''<br />
 
-
==Overview==
+
==CRYSTAL STRUCTURE OF GGA3 GAE DOMAIN IN COMPLEX WITH RABAPTIN-5 PEPTIDE==
-
Adaptor proteins load transmembrane protein cargo into transport vesicles, and serve as nexuses for the formation of large multiprotein complexes on, the nascent vesicles. The gamma-adaptin ear (GAE) domains of the AP-1, adaptor protein complex and the GGA adaptor proteins recruit accessory, proteins to these multiprotein complexes by binding to a hydrophobic, motif. We determined the structure of the GAE domain of human GGA3 in, complex with a peptide based on the DFGPLV sequence of the accessory, protein Rabaptin-5 and refined it at a resolution of 2.2 A. The leucine, and valine residues of the peptide are partly buried in two contiguous, shallow, hydrophobic depressions. The anchoring phenylalanine is buried in, a deep pocket formed by the aliphatic portions of two conserved arginine, residues, along with an alanine and a proline, illustrating the unusual, function of a cluster of basic residues in binding a hydrophobic motif.
+
<StructureSection load='1p4u' size='340' side='right'caption='[[1p4u]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1p4u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P4U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P4U FirstGlance]. <br>
-
1P4U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P4U OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p4u OCA], [https://pdbe.org/1p4u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p4u RCSB], [https://www.ebi.ac.uk/pdbsum/1p4u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p4u ProSAT]</span></td></tr>
-
==Reference==
+
</table>
-
Recognition of accessory protein motifs by the gamma-adaptin ear domain of GGA3., Miller GJ, Mattera R, Bonifacino JS, Hurley JH, Nat Struct Biol. 2003 Aug;10(8):599-606. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12858162 12858162]
+
== Function ==
 +
[https://www.uniprot.org/uniprot/GGA3_HUMAN GGA3_HUMAN] Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.<ref>PMID:11301005</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p4/1p4u_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p4u ConSurf].
 +
<div style="clear:both"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Protein complex]]
+
[[Category: Large Structures]]
-
[[Category: Bonifacino, J.S.]]
+
[[Category: Bonifacino JS]]
-
[[Category: Hurley, J.H.]]
+
[[Category: Hurley JH]]
-
[[Category: Mattera, R.]]
+
[[Category: Mattera R]]
-
[[Category: Miller, G.J.]]
+
[[Category: Miller GJ]]
-
[[Category: protein transport]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:38:49 2008''
+

Current revision

CRYSTAL STRUCTURE OF GGA3 GAE DOMAIN IN COMPLEX WITH RABAPTIN-5 PEPTIDE

PDB ID 1p4u

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1p4u"
Personal tools