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3vou

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[[Image:3vou.jpg|left|200px]]
 
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==The crystal structure of NaK-NavSulP chimera channel==
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The line below this paragraph, containing "STRUCTURE_3vou", creates the "Structure Box" on the page.
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<StructureSection load='3vou' size='340' side='right'caption='[[3vou]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3vou]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_mycoides_KBAB4 Bacillus mycoides KBAB4] and [https://en.wikipedia.org/wiki/Sulfitobacter_sp._NAS-14.1 Sulfitobacter sp. NAS-14.1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VOU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VOU FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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{{STRUCTURE_3vou| PDB=3vou | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vou OCA], [https://pdbe.org/3vou PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vou RCSB], [https://www.ebi.ac.uk/pdbsum/3vou PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vou ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A3SUL8_SULSN A3SUL8_SULSN] [https://www.uniprot.org/uniprot/A9VEV6_BACMK A9VEV6_BACMK]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Most tetrameric channels have cytosolic domains to regulate their functions, including channel inactivation. Here we show that the cytosolic C-terminal region of NavSulP, a prokaryotic voltage-gated sodium channel cloned from Sulfitobacter pontiacus, accelerates channel inactivation. The crystal structure of the C-terminal region of NavSulP grafted into the C-terminus of a NaK channel revealed that the NavSulP C-terminal region forms a four-helix bundle. Point mutations of the residues involved in the intersubunit interactions of the four-helix bundle destabilized the tetramer of the channel and reduced the inactivation rate. The four-helix bundle was directly connected to the inner helix of the pore domain, and a mutation increasing the rigidity of the inner helix also reduced the inactivation rate. These findings suggest that the NavSulP four-helix bundle has important roles not only in stabilizing the tetramer, but also in accelerating the inactivation rate, through promotion of the conformational change of the inner helix.
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===The crystal structure of NaK-NavSulP chimera channel===
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The C-terminal helical bundle of the tetrameric prokaryotic sodium channel accelerates the inactivation rate.,Irie K, Shimomura T, Fujiyoshi Y Nat Commun. 2012 Apr 24;3:793. doi: 10.1038/ncomms1797. PMID:22531178<ref>PMID:22531178</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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[[3vou]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_weihenstephanensis,_sulfitobacter_sp._nas-14.1 Bacillus weihenstephanensis, sulfitobacter sp. nas-14.1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VOU OCA].
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<div class="pdbe-citations 3vou" style="background-color:#fffaf0;"></div>
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[[Category: Bacillus weihenstephanensis, sulfitobacter sp. nas-14 1]]
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== References ==
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[[Category: Fujiyoshi, Y.]]
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<references/>
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[[Category: Irie, K.]]
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__TOC__
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[[Category: Shimomura, T.]]
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</StructureSection>
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[[Category: 4-helical bundle]]
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[[Category: Bacillus mycoides KBAB4]]
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[[Category: Ion channel]]
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[[Category: Large Structures]]
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[[Category: Membrane]]
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[[Category: Sulfitobacter sp. NAS-14 1]]
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[[Category: Transport protein]]
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[[Category: Fujiyoshi Y]]
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[[Category: Irie K]]
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[[Category: Shimomura T]]

Current revision

The crystal structure of NaK-NavSulP chimera channel

PDB ID 3vou

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