3q9o

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[[Image:3q9o.png|left|200px]]
 
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==Full-length Cholix toxin from Vibrio cholerae in complex with NAD==
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The line below this paragraph, containing "STRUCTURE_3q9o", creates the "Structure Box" on the page.
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<StructureSection load='3q9o' size='340' side='right'caption='[[3q9o]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3q9o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q9O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q9O FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.793&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
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{{STRUCTURE_3q9o| PDB=3q9o | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q9o OCA], [https://pdbe.org/3q9o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q9o RCSB], [https://www.ebi.ac.uk/pdbsum/3q9o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q9o ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CHXA_VIBCL CHXA_VIBCL] An NAD-dependent ADP-ribosyltransferase (ADPRT), it catalyzes the transfer of the ADP-ribosyl moiety of oxidized NAD onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. It probably uses the eukaryotic prolow-density lipoprotein receptor-related protein 1 (LRP1) to enter mouse cells, although there seems to be at least one other receptor as well. Is active against mouse fibroblasts, Chinese hamster ovary eEF-2, brine shrimp (Artemia spp. nauplii) and upon expression in S.cerevisiae.<ref>PMID:18276581</ref> <ref>PMID:19793133</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Certain Vibrio cholerae strains produce cholix, a potent protein toxin that has diphthamide-specific ADP-ribosyltransferase activity against eukaryotic elongation factor 2. Here we present a 1.8A crystal structure of cholix in complex with its natural substrate, nicotinamide adenine dinucleotide (NAD+). We also substituted hallmark catalytic residues by site-directed mutagenesis and analyzed both NAD+ binding and ADPribosyltransferase activity using a fluorescence-based assay. These data are the basis for a new kinetic model of cholix toxin activity. Further, the new structural data serve as a reference for continuing inhibitor development for this toxin class.
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===Full-length Cholix toxin from Vibrio cholerae in complex with NAD===
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The 1.8 angstrom cholix toxin crystal structure in complex with NAD and evidence for a new kinetic model.,Fieldhouse RJ, Jorgensen R, Lugo MR, Merrill AR J Biol Chem. 2012 Apr 25. PMID:22535961<ref>PMID:22535961</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3q9o" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_22535961}}, adds the Publication Abstract to the page
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*[[Exotoxin|Exotoxin]]
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(as it appears on PubMed at http://www.pubmed.gov), where 22535961 is the PubMed ID number.
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*[[Exotoxin 3D structures|Exotoxin 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_22535961}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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[[3q9o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q9O OCA].
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[[Category: Large Structures]]
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==Reference==
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<ref group="xtra">PMID:022535961</ref><ref group="xtra">PMID:018276581</ref><references group="xtra"/>
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[[Category: Vibrio cholerae]]
[[Category: Vibrio cholerae]]
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[[Category: Fieldhouse, R J.]]
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[[Category: Fieldhouse RJ]]
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[[Category: Jorgensen, R.]]
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[[Category: Jorgensen R]]
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[[Category: Merrill, A R.]]
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[[Category: Merrill AR]]
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[[Category: Adp-ribosylating factor]]
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[[Category: Alpha-beta complex]]
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[[Category: Beta barrel]]
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[[Category: Diphthamide on eukaryotic elongation factor 2]]
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[[Category: Receptor binding domain]]
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[[Category: Six alpha-helix bundle]]
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[[Category: Transferase]]
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[[Category: Translocation]]
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Current revision

Full-length Cholix toxin from Vibrio cholerae in complex with NAD

PDB ID 3q9o

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