1re0

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[[Image:1re0.jpg|left|200px]]<br /><applet load="1re0" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1re0, resolution 2.40&Aring;" />
 
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'''Structure of ARF1-GDP bound to Sec7 domain complexed with Brefeldin A'''<br />
 
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==Overview==
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==Structure of ARF1-GDP bound to Sec7 domain complexed with Brefeldin A==
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ARF GTPases are activated by guanine nucleotide exchange factors (GEFs) of, the Sec7 family that promote the exchange of GDP for GTP. Brefeldin A, (BFA) is a fungal metabolite that binds to the ARF1*GDP*Sec7 complex and, blocks GEF activity at an early stage of the reaction, prior to guanine, nucleotide release. The crystal structure of the ARF1*GDP*Sec7*BFA complex, shows that BFA binds at the protein-protein interface to inhibit, conformational changes in ARF1 required for Sec7 to dislodge the GDP, molecule. Based on a comparative analysis of the inhibited complex, nucleotide-free ARF1*Sec7 and ARF1*GDP, we suggest that, in addition to, forcing nucleotide release, the ARF1-Sec7 binding energy is used to open a, cavity on ARF1 to facilitate the rearrangement of hydrophobic core, residues between the GDP and GTP conformations. Thus, the Sec7 domain may, act as a dual catalyst, facilitating both nucleotide release and, conformational switching on ARF proteins.
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<StructureSection load='1re0' size='340' side='right'caption='[[1re0]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1re0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RE0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RE0 FirstGlance]. <br>
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1RE0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=GDP:'>GDP</scene>, <scene name='pdbligand=AFB:'>AFB</scene> and <scene name='pdbligand=CIT:'>CIT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RE0 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AFB:1,6,7,8,9,11A,12,13,14,14A-DECAHYDRO-1,13-DIHYDROXY-6-METHYL-4H-CYCLOPENT[F]OXACYCLOTRIDECIN-4-ONE'>AFB</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1re0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1re0 OCA], [https://pdbe.org/1re0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1re0 RCSB], [https://www.ebi.ac.uk/pdbsum/1re0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1re0 ProSAT]</span></td></tr>
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Crystal structure of ARF1*Sec7 complexed with Brefeldin A and its implications for the guanine nucleotide exchange mechanism., Mossessova E, Corpina RA, Goldberg J, Mol Cell. 2003 Dec;12(6):1403-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14690595 14690595]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ARF1_HUMAN ARF1_HUMAN] GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/re/1re0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1re0 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Goldberg, J.]]
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[[Category: Goldberg J]]
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[[Category: Mossessova, E.]]
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[[Category: Mossessova E]]
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[[Category: AFB]]
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[[Category: CIT]]
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[[Category: GDP]]
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[[Category: MG]]
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[[Category: all-helical]]
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[[Category: alph-beta]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:48:15 2008''
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Current revision

Structure of ARF1-GDP bound to Sec7 domain complexed with Brefeldin A

PDB ID 1re0

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