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1rz4

From Proteopedia

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[[Image:1rz4.jpg|left|200px]]<br /><applet load="1rz4" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rz4, resolution 2.1&Aring;" />
 
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'''Crystal Structure of Human eIF3k'''<br />
 
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==Overview==
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==Crystal Structure of Human eIF3k==
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eIF3k, the smallest subunit of eukaryotic initiation factor 3 (eIF3), interacts with several other subunits of eIF3 and the 40 S ribosomal, subunit. eIF3k is conserved among high eukaryotes, including mammals, insects, and plants, and it is ubiquitously expressed in human tissues., Interestingly, eIF3k does not exist in some species of yeast. Thus, eIF3k, may play a unique regulatory role in higher organisms. Here we report the, crystal structure of human eIF3k, the first high-resolution structure of, an eIF3 component. This novel structure contains two distinct domains, a, HEAT (named for Huntington, elongation factor 3, A subunit of protein, phosphatase 2A, target of rapamycin) repeat-like HAM (HEAT analogous, motif) domain and a winged-helix-like WH domain. Through structural, comparison and sequence conservation analysis, we show that eIF3k has, three putative protein-binding surfaces and has potential RNA binding, activity. The structure provides key information for understanding the, structure and function of the eIF3 complex.
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<StructureSection load='1rz4' size='340' side='right'caption='[[1rz4]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rz4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RZ4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz4 OCA], [https://pdbe.org/1rz4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rz4 RCSB], [https://www.ebi.ac.uk/pdbsum/1rz4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rz4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/EIF3K_HUMAN EIF3K_HUMAN] Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rz/1rz4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rz4 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1RZ4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ4 OCA].
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*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of human eIF3k, the first structure of eIF3 subunits., Wei Z, Zhang P, Zhou Z, Cheng Z, Wan M, Gong W, J Biol Chem. 2004 Aug 13;279(33):34983-90. Epub 2004 Jun 4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15180986 15180986]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Gong, W.]]
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[[Category: Gong W]]
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[[Category: Wei, Z.]]
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[[Category: Wei Z]]
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[[Category: Zhang, P.]]
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[[Category: Zhang P]]
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[[Category: Zhou, Z.]]
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[[Category: Zhou Z]]
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[[Category: SO4]]
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[[Category: heat analogous motif]]
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[[Category: winged-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:50:56 2008''
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Current revision

Crystal Structure of Human eIF3k

PDB ID 1rz4

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