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4atu

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(New page: '''Unreleased structure''' The entry 4atu is ON HOLD Authors: Liu, J.S., Schubert, C.R., Fu, X., Fourniol, F.J., Jaiswal, J.K., Houdusse, A., Stultz, C.M., Moores, C.A., Walsh, C.A. De...)
Current revision (10:53, 9 May 2024) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 4atu is ON HOLD
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==Human doublecortin N-DC repeat plus linker, and tubulin (2XRP) docked into an 8A cryo-EM map of doublecortin-stabilised microtubules reconstructed in absence of kinesin==
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<SX load='4atu' size='340' side='right' viewer='molstar' caption='[[4atu]], [[Resolution|resolution]] 8.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4atu]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ATU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ATU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 8.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4atu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4atu OCA], [https://pdbe.org/4atu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4atu RCSB], [https://www.ebi.ac.uk/pdbsum/4atu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4atu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TBB2B_BOVIN TBB2B_BOVIN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Doublecortin (Dcx) defines a growing family of microtubule (MT)-associated proteins (MAPs) involved in neuronal migration and process outgrowth. We show that Dcx is essential for the function of Kif1a, a kinesin-3 motor protein that traffics synaptic vesicles. Neurons lacking Dcx and/or its structurally conserved paralogue, doublecortin-like kinase 1 (Dclk1), show impaired Kif1a-mediated transport of Vamp2, a cargo of Kif1a, with decreased run length. Human disease-associated mutations in Dcx's linker sequence (e.g., W146C, K174E) alter Kif1a/Vamp2 transport by disrupting Dcx/Kif1a interactions without affecting Dcx MT binding. Dcx specifically enhances binding of the ADP-bound Kif1a motor domain to MTs. Cryo-electron microscopy and subnanometer-resolution image reconstruction reveal the kinesin-dependent conformational variability of MT-bound Dcx and suggest a model for MAP-motor crosstalk on MTs. Alteration of kinesin run length by MAPs represents a previously undiscovered mode of control of kinesin transport and provides a mechanism for regulation of MT-based transport by local signals.
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Authors: Liu, J.S., Schubert, C.R., Fu, X., Fourniol, F.J., Jaiswal, J.K., Houdusse, A., Stultz, C.M., Moores, C.A., Walsh, C.A.
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Molecular basis for specific regulation of neuronal Kinesin-3 motors by doublecortin family proteins.,Liu JS, Schubert CR, Fu X, Fourniol FJ, Jaiswal JK, Houdusse A, Stultz CM, Moores CA, Walsh CA Mol Cell. 2012 Sep 14;47(5):707-21. doi: 10.1016/j.molcel.2012.06.025. Epub 2012 , Aug 1. PMID:22857951<ref>PMID:22857951</ref>
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Description: Human doublecortin N-DC repeat plus linker, and tubulin (2XRP) docked into an 8A cryo-EM map of doublecortin-stabilised microtubules reconstructed in absence of kinesin
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4atu" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Tubulin 3D Structures|Tubulin 3D Structures]]
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== References ==
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<references/>
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__TOC__
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</SX>
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[[Category: Bos taurus]]
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Fourniol FJ]]
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[[Category: Fu X]]
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[[Category: Houdusse A]]
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[[Category: Jaiswal JK]]
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[[Category: Liu JS]]
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[[Category: Moores CA]]
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[[Category: Schubert CR]]
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[[Category: Stultz CM]]
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[[Category: Walsh CA]]

Current revision

Human doublecortin N-DC repeat plus linker, and tubulin (2XRP) docked into an 8A cryo-EM map of doublecortin-stabilised microtubules reconstructed in absence of kinesin

4atu, resolution 8.30Å

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