3s5m
From Proteopedia
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| - | [[Image:3s5m.jpg|left|200px]] | ||
| - | + | ==Crystal structures of falcilysin, a M16 metalloprotease from the malaria parasite Plasmodium falciparum== | |
| - | + | <StructureSection load='3s5m' size='340' side='right'caption='[[3s5m]], [[Resolution|resolution]] 1.55Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[3s5m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_HB3 Plasmodium falciparum HB3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S5M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S5M FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55Å</td></tr> | |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s5m OCA], [https://pdbe.org/3s5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s5m RCSB], [https://www.ebi.ac.uk/pdbsum/3s5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s5m ProSAT]</span></td></tr> | |
| - | + | </table> | |
| - | + | == Function == | |
| - | + | [https://www.uniprot.org/uniprot/FCLN_PLAF7 FCLN_PLAF7] In the food vacuole, acts downstream of proteases plasmepsins PMI and PMII and falcipains during the catabolism of host hemoglobin by cleaving peptide fragments of alpha and beta hemoglobin subunits generated by PMI and PMII and falcipains (PubMed:17074076). In the apicoplast, degrades apicoplast transit peptides after their cleavage (PubMed:17074076). Prefers bulky hydrophobic amino acids in the P1' position at both acidic and neutral pH (By similarity). At P2', prefers hydrophobic residues at acidic pH; at neutral pH, these same residues are abundant but prefers Arg (By similarity). At P3', prefers hydrophobic residues, especially Met, at both pH conditions. At P4' and P5', prefers acidic residues at acidic pH, however, at neutral pH, the enzyme is less selective at these positions (By similarity). The optimal site cleavage at acidic pH is YNEHS-|-FFMEE and, at neutral pH, MKRHS-|-FRMRG (By similarity).[UniProtKB:A0A0L7KF24]<ref>PMID:17074076</ref> | |
| - | + | == References == | |
| - | == | + | <references/> |
| - | [[3s5m]] is a 1 chain structure with sequence from [ | + | __TOC__ |
| - | [[ | + | </StructureSection> |
| - | [[ | + | [[Category: Large Structures]] |
| - | [[ | + | [[Category: Plasmodium falciparum HB3]] |
| - | [ | + | [[Category: Eneqvist T]] |
| - | [[Category: | + | [[Category: Goldberg D]] |
| - | [[Category: | + | [[Category: Istvan E]] |
| - | [[Category: | + | [[Category: Morgunova E]] |
| - | [[Category: | + | [[Category: Ponpuak M]] |
| - | [[Category: | + | [[Category: Popov A]] |
| - | [[Category: | + | |
Current revision
Crystal structures of falcilysin, a M16 metalloprotease from the malaria parasite Plasmodium falciparum
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