3vpk
From Proteopedia
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| - | [[Image:3vpk.jpg|left|200px]] | ||
| - | < | + | ==Crystal Structure of 6-Guanidinohexanoyl Trypsin== |
| - | + | <StructureSection load='3vpk' size='340' side='right'caption='[[3vpk]], [[Resolution|resolution]] 1.94Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[3vpk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VPK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VPK FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94Å</td></tr> | |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=E4B:ETHYL+4-HYDROXYBENZOATE'>E4B</scene>, <scene name='pdbligand=GHS:6-CARBAMIMIDAMIDOHEXANOIC+ACID'>GHS</scene></td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vpk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vpk OCA], [https://pdbe.org/3vpk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vpk RCSB], [https://www.ebi.ac.uk/pdbsum/3vpk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vpk ProSAT]</span></td></tr> | |
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/TRY1_BOVIN TRY1_BOVIN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The force driving the conversion from the acyl intermediate to the tetrahedral intermediate in the deacylation reaction of serine proteases remains unclear. The crystal structure of 6-guanidinohexanoyl trypsin was determined at pH 7.0, near the optimum reaction pH, at 1.94 A resolution. In this structure, three water molecules are observed around the catalytic site. One acts as a nucleophile to attack the acyl carbonyl carbon while the other two waters fix the position of the catalytic water through a hydrogen bond. When the acyl carbonyl oxygen oscillates thermally, the water assumes an appropriate angle to catalyze the deacylation. Proteins 2013. (c) 2012 Wiley Periodicals, Inc. | ||
| - | + | Crystal structure of 6-guanidinohexanoyl trypsin near the optimum pH reveals the acyl-enzyme intermediate to be deacylated.,Masuda Y, Nitanai Y, Mizutani R, Noguchi S Proteins. 2013 Mar;81(3):526-30. doi: 10.1002/prot.24206. Epub 2012 Dec 24. PMID:23161653<ref>PMID:23161653</ref> | |
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3vpk" style="background-color:#fffaf0;"></div> | ||
| - | == | + | ==See Also== |
| - | [[ | + | *[[Trypsin 3D structures|Trypsin 3D structures]] |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Masuda | + | [[Category: Masuda Y]] |
| - | [[Category: Mizutani | + | [[Category: Mizutani R]] |
| - | [[Category: Nitanai | + | [[Category: Nitanai Y]] |
| - | [[Category: Noguchi | + | [[Category: Noguchi S]] |
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Current revision
Crystal Structure of 6-Guanidinohexanoyl Trypsin
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