2rse

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (20:52, 12 April 2023) (edit) (undo)
 
(4 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2rse.jpg|left|200px]]
 
-
<!--
+
==NMR structure of FKBP12-mTOR FRB domain-rapamycin complex structure determined based on PCS==
-
The line below this paragraph, containing "STRUCTURE_2rse", creates the "Structure Box" on the page.
+
<StructureSection load='2rse' size='340' side='right'caption='[[2rse]]' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2rse]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RSE FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TB:TERBIUM(III)+ION'>TB</scene></td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rse OCA], [https://pdbe.org/2rse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rse RCSB], [https://www.ebi.ac.uk/pdbsum/2rse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rse ProSAT]</span></td></tr>
-
{{STRUCTURE_2rse| PDB=2rse | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/FKB1A_HUMAN FKB1A_HUMAN] Keeps in an inactive conformation TGFBR1, the TGF-beta type I serine/threonine kinase receptor, preventing TGF-beta receptor activation in absence of ligand. Recruites SMAD7 to ACVR1B which prevents the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. May modulate the RYR1 calcium channel activity. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.<ref>PMID:9233797</ref> <ref>PMID:16720724</ref>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Pseudo contact shifts (PCSs) induced by paramagnetic lanthanide ions fixed in a protein frame provide long-range distance and angular information, and are valuable for the structure determination of protein-protein and protein-ligand complexes. We have been developing a lanthanide-binding peptide tag (hereafter LBT) anchored at two points via a peptide bond and a disulfide bond to the target proteins. However, the magnetic susceptibility tensor displays symmetry, which can cause multiple degenerated solutions in a structure calculation based solely on PCSs. Here we show a convenient method for resolving this degeneracy by changing the spacer length between the LBT and target protein. We applied this approach to PCS-based rigid body docking between the FKBP12-rapamycin complex and the mTOR FRB domain, and demonstrated that degeneracy could be resolved using the PCS restraints obtained from two-point anchored LBT with two different spacer lengths. The present strategy will markedly increase the usefulness of two-point anchored LBT for protein complex structure determination.
-
===NMR structure of FKBP12-mTOR FRB domain-rapamycin complex structure determined based on PCS===
+
Convenient method for resolving degeneracies due to symmetry of the magnetic susceptibility tensor and its application to pseudo contact shift-based protein-protein complex structure determination.,Kobashigawa Y, Saio T, Ushio M, Sekiguchi M, Yokochi M, Ogura K, Inagaki F J Biomol NMR. 2012 May;53(1):53-63. Epub 2012 Apr 10. PMID:22487935<ref>PMID:22487935</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2rse" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_22487935}}, adds the Publication Abstract to the page
+
*[[FKBP 3D structures|FKBP 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 22487935 is the PubMed ID number.
+
*[[Serine/threonine protein kinase 3D structures|Serine/threonine protein kinase 3D structures]]
-
-->
+
== References ==
-
{{ABSTRACT_PUBMED_22487935}}
+
<references/>
-
 
+
__TOC__
-
==About this Structure==
+
</StructureSection>
-
[[2rse]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RSE OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:022487935</ref><references group="xtra"/>
+
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Peptidylprolyl isomerase]]
+
[[Category: Large Structures]]
-
[[Category: Inagaki, F.]]
+
[[Category: Inagaki F]]
-
[[Category: Kobashigawa, Y.]]
+
[[Category: Kobashigawa Y]]
-
[[Category: Saio, T.]]
+
[[Category: Saio T]]
-
[[Category: Ushio, M.]]
+
[[Category: Ushio M]]
-
[[Category: Fk506]]
+
-
[[Category: Fkbp12]]
+
-
[[Category: Isomerase-transferase complex]]
+
-
[[Category: Lanthanide]]
+
-
[[Category: Pc]]
+
-
[[Category: Rapamycin]]
+

Current revision

NMR structure of FKBP12-mTOR FRB domain-rapamycin complex structure determined based on PCS

PDB ID 2rse

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2rse"
Personal tools