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2j6p

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[[Image:2j6p.png|left|200px]]
 
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==STRUCTURE OF AS-SB REDUCTASE FROM LEISHMANIA MAJOR==
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The line below this paragraph, containing "STRUCTURE_2j6p", creates the "Structure Box" on the page.
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<StructureSection load='2j6p' size='340' side='right'caption='[[2j6p]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2j6p]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J6P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J6P FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2j6p| PDB=2j6p | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j6p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j6p OCA], [https://pdbe.org/2j6p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j6p RCSB], [https://www.ebi.ac.uk/pdbsum/2j6p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j6p ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q6Q1Q5_LEIMA Q6Q1Q5_LEIMA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j6/2j6p_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j6p ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The arsenate/antimonate reductase LmACR2 has been recently identified in the genome of Leishmania major. Besides displaying phosphatase activity in vitro, this enzyme is able to reduce both As(V) and Sb(V) to their respective trivalent forms and is involved in the activation of Pentostan, a drug containing Sb(V) used in the treatment of leishmaniasis. LmACR2 displays sequence and functional similarity with the arsenate reductase ScACR2 from Saccharomyces cerevisiae, and both proteins are homologous to the catalytic domain of Cdc25 phosphatases, which, in turn, belong to the rhodanese/Cdc25 phosphatase superfamily. In this work, the three-dimensional structure of LmACR2 has been determined with crystallographic methods and refined at 2.15 A resolution. The protein structure maintains the overall rhodanese fold, but substantial modifications are observed in secondary structure position and length. However, the conformation of the active-site loop and the position of the catalytic residue Cys75 are unchanged with respect to the Cdc25 phosphatases. From an evolutionary viewpoint, LmACR2 and the related arsenate reductases form, together with the known Cdc25 phosphatases, a well-defined subfamily of the rhodanese/Cdc25 phosphatase superfamily, characterized by a 7-amino-acid-long active-site loop that is able to selectively bind substrates containing phosphorous, arsenic, or antinomy. The evolutionary tree obtained for these proteins shows that, besides the active-site motif CE[F/Y]SXXR that characterizes Cdc25 phosphatase, the novel CALSQ[Q/V]R motif is also conserved in sequences from fungi and plants. Similar to Cdc25 phosphatase, these proteins are likely involved in cell cycle control. The active-site composition of LmACR2 (CAQSLVR) does not belong to either group, but gives to the enzyme a bifunctional activity of both phosphatase and As/Sb reductase. The subtle dependence of substrate specificity on the amino acid composition of the active-site loop displays the versatility of the ubiquitous rhodanese domain.
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===STRUCTURE OF AS-SB REDUCTASE FROM LEISHMANIA MAJOR===
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Structural characterization of the As/Sb reductase LmACR2 from Leishmania major.,Mukhopadhyay R, Bisacchi D, Zhou Y, Armirotti A, Bordo D J Mol Biol. 2009 Mar 13;386(5):1229-39. Epub 2008 Jul 29. PMID:18687336<ref>PMID:18687336</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18687336}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2j6p" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18687336 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18687336}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[2j6p]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J6P OCA].
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==Reference==
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<ref group="xtra">PMID:018687336</ref><references group="xtra"/>
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[[Category: Leishmania major]]
[[Category: Leishmania major]]
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[[Category: Bisacchi, D.]]
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[[Category: Bisacchi D]]
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[[Category: Bordo, D.]]
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[[Category: Bordo D]]
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[[Category: Mukhopadhyay, R.]]
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[[Category: Mukhopadhyay R]]
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[[Category: Rosen, B P.]]
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[[Category: Rosen BP]]
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[[Category: Zhou, Y.]]
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[[Category: Zhou Y]]
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[[Category: Antimonate reductase]]
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[[Category: Arsenate reductase]]
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[[Category: C-myc epitope]]
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[[Category: Cdc25 phosphatase]]
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[[Category: Oxidoreductase]]
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[[Category: Rhodanese]]
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Current revision

STRUCTURE OF AS-SB REDUCTASE FROM LEISHMANIA MAJOR

PDB ID 2j6p

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