3e1z

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[[Image:3e1z.png|left|200px]]
 
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==Crystal structure of the parasite protesase inhibitor chagasin in complex with papain==
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The line below this paragraph, containing "STRUCTURE_3e1z", creates the "Structure Box" on the page.
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<StructureSection load='3e1z' size='340' side='right'caption='[[3e1z]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3e1z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Carica_papaya Carica papaya] and [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E1Z FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.86&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_3e1z| PDB=3e1z | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e1z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e1z OCA], [https://pdbe.org/3e1z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e1z RCSB], [https://www.ebi.ac.uk/pdbsum/3e1z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e1z ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CHAG_TRYCR CHAG_TRYCR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e1/3e1z_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3e1z ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A complex of chagasin, a protein inhibitor from Trypanosoma cruzi, and papain, a classic family C1 cysteine protease, has been crystallized. Kinetic studies revealed that inactivation of papain by chagasin is very fast (k(on) = 1.5 x 10(6) M(-1) x s(-1)), and results in the formation of a very tight, reversible complex (K(i) = 36 pM), with similar or better rate and equilibrium constants than those for cathepsins L and B. The high-resolution crystal structure shows an inhibitory wedge comprising three loops, which forms a number of contacts responsible for the high-affinity binding. Comparison with the structure of papain in complex with human cystatin B reveals that, despite entirely different folding, the two inhibitors utilize very similar atomic interactions, leading to essentially identical affinities for the enzyme. Comparisons of the chagasin-papain complex with high-resolution structures of chagasin in complexes with cathepsin L, cathepsin B and falcipain allowed the creation of a consensus map of the structural features that are important for efficient inhibition of papain-like enzymes. The comparisons also revealed a number of unique interactions that can be used to design enzyme-specific inhibitors. As papain exhibits high structural similarity to the catalytic domain of the T. cruzi enzyme cruzipain, the present chagasin-papain complex provides a reliable model of chagasin-cruzipain interactions. Such information, coupled with our identification of specificity-conferring interactions, should be important for the development of drugs for treatment of the devastating Chagas disease caused by this parasite.
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===Crystal structure of the parasite protesase inhibitor chagasin in complex with papain===
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Crystal structure of the parasite inhibitor chagasin in complex with papain allows identification of structural requirements for broad reactivity and specificity determinants for target proteases.,Redzynia I, Ljunggren A, Bujacz A, Abrahamson M, Jaskolski M, Bujacz G FEBS J. 2009 Feb;276(3):793-806. PMID:19143838<ref>PMID:19143838</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3e1z" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19143838 is the PubMed ID number.
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{{ABSTRACT_PUBMED_19143838}}
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==About this Structure==
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[[3e1z]] is a 2 chain structure of [[Papain]] with sequence from [http://en.wikipedia.org/wiki/Carica_papaya Carica papaya] and [http://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1Z OCA].
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==See Also==
==See Also==
*[[Papain|Papain]]
*[[Papain|Papain]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019143838</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Carica papaya]]
[[Category: Carica papaya]]
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[[Category: Papain]]
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[[Category: Large Structures]]
[[Category: Trypanosoma cruzi]]
[[Category: Trypanosoma cruzi]]
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[[Category: Abrahamson, M.]]
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[[Category: Abrahamson M]]
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[[Category: Bujacz, A.]]
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[[Category: Bujacz A]]
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[[Category: Bujacz, G.]]
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[[Category: Bujacz G]]
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[[Category: Jaskolski, M.]]
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[[Category: Jaskolski M]]
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[[Category: Ljunggren, A.]]
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[[Category: Ljunggren A]]
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[[Category: Redzynia, I.]]
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[[Category: Redzynia I]]
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[[Category: Allergen]]
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[[Category: Chagas disease]]
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[[Category: Chagasin-papain complex]]
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[[Category: Cysteine proteinase]]
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[[Category: Cytoplasmic vesicle]]
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[[Category: Hydrolase]]
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[[Category: Hydrolase inhibitor-hydrolase complex]]
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[[Category: Papain]]
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[[Category: Protease]]
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[[Category: Protease inhibitor]]
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[[Category: Protein inhibitor]]
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[[Category: Thiol protease]]
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[[Category: Thiol protease inhibitor]]
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[[Category: Zymogen]]
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Current revision

Crystal structure of the parasite protesase inhibitor chagasin in complex with papain

PDB ID 3e1z

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