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1tc1

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[[Image:1tc1.png|left|200px]]
 
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==A 1.4 ANGSTROM CRYSTAL STRUCTURE FOR THE HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE OF TRYPANOSOMA CRUZI==
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The line below this paragraph, containing "STRUCTURE_1tc1", creates the "Structure Box" on the page.
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<StructureSection load='1tc1' size='340' side='right'caption='[[1tc1]], [[Resolution|resolution]] 1.41&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1tc1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TC1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TC1 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.41&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMB:FORMYCIN+B'>FMB</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
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{{STRUCTURE_1tc1| PDB=1tc1 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tc1 OCA], [https://pdbe.org/1tc1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tc1 RCSB], [https://www.ebi.ac.uk/pdbsum/1tc1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tc1 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q4DRC4_TRYCC Q4DRC4_TRYCC]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tc/1tc1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tc1 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The hypoxanthine phosphoribosyltransferase (HPRT) from Trypanosoma cruzi, etiologic agent of Chagas' disease, was cocrystallized with the inosine analogue Formycin B (FmB) and the structure determined to 1.4 A resolution. This is the highest resolution structure yet reported for a phosphoribosyltransferase (PRT), and the asymmetric unit of the crystal contains a dimer of closely associated, nearly identical subunits. A conserved nonproline cis peptide in one active-site loop exposes the main-chain nitrogen to the enzyme active site, while the adjacent lysine side chain interacts with the other subunit of the dimer, thereby providing a possible mechanism for communication between the subunits and their active sites. The three-dimensional coordinates for the invariant Ser103-Tyr104 dipeptide are reported here for the first time. These are the only highly conserved residues in a second active-site loop, termed the long flexible loop, which is predicted to close over the active site of HPRTs to protect a labile transition state [Eads et al. (1994) Cell 78, 325-334]. This structure represents a major step forward in efforts to design/discover potent selective inhibitors of the HPRT of T. cruzi.
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===A 1.4 ANGSTROM CRYSTAL STRUCTURE FOR THE HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE OF TRYPANOSOMA CRUZI===
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A 1.4 A crystal structure for the hypoxanthine phosphoribosyltransferase of Trypanosoma cruzi.,Focia PJ, Craig SP 3rd, Nieves-Alicea R, Fletterick RJ, Eakin AE Biochemistry. 1998 Oct 27;37(43):15066-75. PMID:9790669<ref>PMID:9790669</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1tc1" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_9790669}}, adds the Publication Abstract to the page
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*[[Phosphoribosyltransferase 3D structures|Phosphoribosyltransferase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 9790669 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_9790669}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[1tc1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TC1 OCA].
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==Reference==
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<ref group="xtra">PMID:009790669</ref><references group="xtra"/>
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[[Category: Hypoxanthine phosphoribosyltransferase]]
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[[Category: Trypanosoma cruzi]]
[[Category: Trypanosoma cruzi]]
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[[Category: Eakin, A E.]]
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[[Category: Craig III SP]]
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[[Category: Fletterick, R J.]]
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[[Category: Eakin AE]]
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[[Category: Focia, P J.]]
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[[Category: Fletterick RJ]]
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[[Category: III, S P.Craig.]]
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[[Category: Focia PJ]]
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[[Category: Nieves-Alicea, R.]]
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[[Category: Nieves-Alicea R]]
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[[Category: Nucleotide metabolism]]
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[[Category: Phosphoribosyltransferase]]
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[[Category: Purine salvage]]
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[[Category: Transferase]]
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Current revision

A 1.4 ANGSTROM CRYSTAL STRUCTURE FOR THE HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE OF TRYPANOSOMA CRUZI

PDB ID 1tc1

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