3f5m

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[[Image:3f5m.png|left|200px]]
 
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==Crystal Structure of ATP-Bound Phosphofructokinase from Trypanosoma brucei==
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<StructureSection load='3f5m' size='340' side='right'caption='[[3f5m]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3f5m]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F5M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3F5M FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3f5m| PDB=3f5m | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3f5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f5m OCA], [https://pdbe.org/3f5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3f5m RCSB], [https://www.ebi.ac.uk/pdbsum/3f5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3f5m ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PFKA_TRYBB PFKA_TRYBB] Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.[HAMAP-Rule:MF_03186]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of the ATP-bound form of the tetrameric phosphofructokinase (PFK) from Trypanosoma brucei enables detailed comparisons to be made with the structures of the apoenzyme form of the same enzyme, as well as with those of bacterial ATP-dependent and PP(i)-dependent PFKs. The active site of T. brucei PFK (which is strictly ATP-dependent but belongs to the PP(i)-dependent family by sequence similarities) is a chimera of the two types of PFK. In particular, the active site of T. brucei PFK possesses amino acid residues and structural features characteristic of both types of PFK. Conformational changes upon ATP binding are observed that include the opening of the active site to accommodate the two substrates, MgATP and fructose 6-phosphate, and a dramatic ordering of the C-terminal helices, which act like reaching arms to hold the tetramer together. These conformational transitions are fundamentally different from those of other ATP-dependent PFKs. The substantial differences in structure and mechanism of T. brucei PFK compared with bacterial and mammalian PFKs give optimism for the discovery of species-specific drugs for the treatment of diseases caused by protist parasites of the trypanosomatid family.
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===Crystal Structure of ATP-Bound Phosphofructokinase from Trypanosoma brucei===
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The crystal structure of ATP-bound phosphofructokinase from Trypanosoma brucei reveals conformational transitions different from those of other phosphofructokinases.,McNae IW, Martinez-Oyanedel J, Keillor JW, Michels PA, Fothergill-Gilmore LA, Walkinshaw MD J Mol Biol. 2009 Feb 6;385(5):1519-33. Epub 2008 Dec 3. PMID:19084537<ref>PMID:19084537</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 3f5m" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19084537 is the PubMed ID number.
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{{ABSTRACT_PUBMED_19084537}}
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==About this Structure==
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[[3f5m]] is a 4 chain structure of [[Phosphofructokinase (PFK)]] with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F5M OCA].
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==See Also==
==See Also==
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*[[Phosphofructokinase (PFK)|Phosphofructokinase (PFK)]]
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*[[Phosphofructokinase 3D structures|Phosphofructokinase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019084537</ref><references group="xtra"/>
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__TOC__
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[[Category: 6-phosphofructokinase]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Trypanosoma brucei]]
[[Category: Trypanosoma brucei]]
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[[Category: Fothergill-Gilmore, L A.]]
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[[Category: Fothergill-Gilmore LA]]
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[[Category: Keillor, J W.]]
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[[Category: Keillor JW]]
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[[Category: Martinez-Oyanedel, J.]]
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[[Category: Martinez-Oyanedel J]]
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[[Category: McNae, I W.]]
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[[Category: McNae IW]]
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[[Category: Michels, P A.M.]]
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[[Category: Michels PAM]]
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[[Category: Walkinshaw, M D.]]
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[[Category: Walkinshaw MD]]
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[[Category: 6-phospho-1-fructokinase]]
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[[Category: Atp binding]]
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[[Category: Atp-binding]]
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[[Category: Glycolysis]]
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[[Category: Kinase]]
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[[Category: Kinase activity]]
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[[Category: Nucleotide-binding]]
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[[Category: Transferase]]
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Current revision

Crystal Structure of ATP-Bound Phosphofructokinase from Trypanosoma brucei

PDB ID 3f5m

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