1p33

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[[Image:1p33.png|left|200px]]
 
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==Pteridine reductase from Leishmania tarentolae complex with NADPH and MTX==
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The line below this paragraph, containing "STRUCTURE_1p33", creates the "Structure Box" on the page.
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<StructureSection load='1p33' size='340' side='right'caption='[[1p33]], [[Resolution|resolution]] 2.86&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1p33]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_tarentolae Leishmania tarentolae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P33 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P33 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.86&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MTX:METHOTREXATE'>MTX</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
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{{STRUCTURE_1p33| PDB=1p33 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p33 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p33 OCA], [https://pdbe.org/1p33 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p33 RCSB], [https://www.ebi.ac.uk/pdbsum/1p33 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p33 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PTR1_LEITA PTR1_LEITA] Exhibits a NADPH-dependent biopterin reductase activity. Has good activity with folate and significant activity with dihydrofolate and dihydrobiopterin, but not with quinonoid dihydrobiopterin. Confers resistance to methotrexate (MTX).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p3/1p33_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p33 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The protozoan parasites Leishmania utilize a pteridine-reducing enzyme, pteridine reductase (PTR1), to bypass antifolate inhibition. The crystal structure of PTR1 from L. tarentolae has been solved as a binary complex with NADPH at 2.8 A resolution. The structure was solved by molecular-replacement techniques using the recently reported L. major PTR1 structure as a search model. Comparisons of the present structure with the L. major PTR1 allowed us to identify regions of flexibility in the molecule. PTR1 is a member of the growing family of short-chain dehydrogenases (SDR) which share the characteristic Tyr(Xaa)(3)Lys motif in the vicinity of the active site. The functional enzyme is a tetramer and the crystallographic asymmetric unit contains a tetramer with 222 point-group symmetry.
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===Pteridine reductase from Leishmania tarentolae complex with NADPH and MTX===
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Structure of pteridine reductase (PTR1) from Leishmania tarentolae.,Zhao H, Bray T, Ouellette M, Zhao M, Ferre RA, Matthews D, Whiteley JM, Varughese KI Acta Crystallogr D Biol Crystallogr. 2003 Sep;59(Pt 9):1539-44. Epub 2003, Aug 19. PMID:12925782<ref>PMID:12925782</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1p33" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_12925782}}, adds the Publication Abstract to the page
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*[[Pteridine reductase|Pteridine reductase]]
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(as it appears on PubMed at http://www.pubmed.gov), where 12925782 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12925782}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[1p33]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_tarentolae Leishmania tarentolae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P33 OCA].
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==Reference==
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<ref group="xtra">PMID:012925782</ref><references group="xtra"/>
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[[Category: Leishmania tarentolae]]
[[Category: Leishmania tarentolae]]
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[[Category: Pteridine reductase]]
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[[Category: Bray T]]
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[[Category: Bray, T.]]
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[[Category: Ferre RA]]
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[[Category: Ferre, R A.]]
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[[Category: Matthews D]]
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[[Category: Matthews, D.]]
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[[Category: Ouellette M]]
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[[Category: Ouellette, M.]]
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[[Category: Varughese KI]]
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[[Category: Varughese, K I.]]
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[[Category: Whiteley JM]]
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[[Category: Whiteley, J M.]]
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[[Category: Zhao H]]
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[[Category: Zhao, H.]]
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[[Category: Zhao M]]
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[[Category: Zhao, M.]]
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[[Category: Complex]]
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[[Category: Mtx]]
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[[Category: Nadph]]
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[[Category: Oxidoreductase]]
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[[Category: Pteridine reductase]]
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Current revision

Pteridine reductase from Leishmania tarentolae complex with NADPH and MTX

PDB ID 1p33

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OCA

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