2vwe

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[[Image:2vwe.png|left|200px]]
 
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==Crystal Structure of Vascular Endothelial Growth Factor-B in Complex with a Neutralizing Antibody Fab Fragment==
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The line below this paragraph, containing "STRUCTURE_2vwe", creates the "Structure Box" on the page.
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<StructureSection load='2vwe' size='340' side='right'caption='[[2vwe]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2vwe]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VWE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VWE FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vwe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vwe OCA], [https://pdbe.org/2vwe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vwe RCSB], [https://www.ebi.ac.uk/pdbsum/2vwe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vwe ProSAT]</span></td></tr>
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{{STRUCTURE_2vwe| PDB=2vwe | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VEGFB_HUMAN VEGFB_HUMAN] Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vw/2vwe_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vwe ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Vascular endothelial growth factor (VEGF) B effects blood vessel formation by binding to VEGF receptor 1. To study the specifics of the biological profile of VEGF-B in both physiological and pathological angiogenesis, a neutralising anti-VEGF-B antibody (2H10) that functions by inhibiting the binding of VEGF-B to VEGF receptor 1 was developed. Here, we present the structural features of the 'highly ordered' interaction of the Fab fragment of this antibody (Fab-2H10) with VEGF-B. Two molecules of Fab-2H10 bind to symmetrical binding sites located at each pole of the VEGF-B homodimer, giving a unique U-shaped topology to the complex that has not been previously observed in the VEGF family. VEGF-B residues essential for binding to the antibody are contributed by both monomers of the cytokine. Our detailed analysis reveals that the neutralising effect of the antibody occurs by virtue of the steric hindrance of the receptor-binding interface. These findings suggest that functional complementarity between VEGF-B and 2H10 can be harnessed both in analysing the therapeutic potential of VEGF-B and as an antagonist of receptor activation.
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===CRYSTAL STRUCTURE OF VASCULAR ENDOTHELIAL GROWTH FACTOR-B IN COMPLEX WITH A NEUTRALIZING ANTIBODY FAB FRAGMENT===
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Crystal structure of vascular endothelial growth factor-B in complex with a neutralising antibody Fab fragment.,Leonard P, Scotney PD, Jabeen T, Iyer S, Fabri LJ, Nash AD, Acharya KR J Mol Biol. 2008 Dec 31;384(5):1203-17. Epub 2008 Oct 9. PMID:18930733<ref>PMID:18930733</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18930733}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2vwe" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18930733 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18930733}}
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==About this Structure==
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[[2vwe]] is a 6 chain structure of [[Vascular Endothelial Growth Factor]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VWE OCA].
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==See Also==
==See Also==
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*[[Vascular Endothelial Growth Factor|Vascular Endothelial Growth Factor]]
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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*[[VEGF 3D Structures|VEGF 3D Structures]]
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==Reference==
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== References ==
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<ref group="xtra">PMID:018930733</ref><references group="xtra"/>
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Acharya, K R.]]
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[[Category: Acharya KR]]
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[[Category: Fabri, L J.]]
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[[Category: Fabri LJ]]
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[[Category: Iyer, S.]]
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[[Category: Iyer S]]
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[[Category: Jabeen, T.]]
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[[Category: Jabeen T]]
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[[Category: Leonard, P.]]
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[[Category: Leonard P]]
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[[Category: Nash, A D.]]
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[[Category: Nash AD]]
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[[Category: Scotney, P D.]]
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[[Category: Scotney PD]]
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[[Category: Angiogenesis]]
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[[Category: Cysteine-knot]]
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[[Category: Glycoprotein]]
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[[Category: Growth factor]]
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[[Category: Heparin-binding]]
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[[Category: Immune system]]
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[[Category: Mitogen]]
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[[Category: Neutralizing antibody]]
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[[Category: Secreted]]
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[[Category: Vegf-b]]
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Current revision

Crystal Structure of Vascular Endothelial Growth Factor-B in Complex with a Neutralizing Antibody Fab Fragment

PDB ID 2vwe

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