1hhq

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px <!-- The line below this paragraph, containing "STRUCTURE_1hhq", creates the "Structure Box" on the page. You may change the PDB parameter (which sets the PD...)
Current revision (12:26, 13 December 2023) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1hhq.png|left|200px]]
 
-
<!--
+
==Role of active site resiude Lys16 in Nucleoside Diphosphate Kinase==
-
The line below this paragraph, containing "STRUCTURE_1hhq", creates the "Structure Box" on the page.
+
<StructureSection load='1hhq' size='340' side='right'caption='[[1hhq]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1hhq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HHQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HHQ FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
{{STRUCTURE_1hhq| PDB=1hhq | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hhq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hhq OCA], [https://pdbe.org/1hhq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hhq RCSB], [https://www.ebi.ac.uk/pdbsum/1hhq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hhq ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/NDKC_DICDI NDKC_DICDI]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hh/1hhq_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hhq ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Nucleoside diphosphate (NDP) kinase phosphorylates nucleoside diphosphates with little specificity for the base and the sugar. Although nucleotide analogues used in antiviral therapies are also metabolized to their triphosphate form by NDP kinase, their lack of the 3'-hydroxyl of the ribose, which allows them to be DNA chain terminators, severely impairs the catalytic efficiency of NDP kinase. We have analyzed the kinetics parameters of several mutant NDP kinases modified on residues (Lys16, Tyr56, Asn119) interacting with the gamma-phosphate and/or the 3'-OH of the Mg2+-ATP substrate. We compared the relative contributions of the active-site residues and the substrate 3'-OH for point mutations on Lys16, Tyr56 and Asn119. Analysis of additional data from pH profiles identify the ionization state of these residues in the enzyme active form. X-ray structure of K16A mutant NDP kinase shows no detectable rearrangement of the residues of the active site.
-
===ROLE OF ACTIVE SITE RESIUDE LYS16 IN NUCLEOSIDE DIPHOSPHATE KINASE===
+
Mechanism of phosphoryl transfer by nucleoside diphosphate kinase pH dependence and role of the active site Lys16 and Tyr56 residues.,Schneider B, Babolat M, Xu YW, Janin J, Veron M, Deville-Bonne D Eur J Biochem. 2001 Apr;268(7):1964-71. PMID:11277918<ref>PMID:11277918</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_11277918}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 1hhq" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 11277918 is the PubMed ID number.
+
-
-->
+
-
{{ABSTRACT_PUBMED_11277918}}
+
-
 
+
-
==About this Structure==
+
-
[[1hhq]] is a 1 chain structure of [[Nucleoside diphosphate kinase]] with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HHQ OCA].
+
==See Also==
==See Also==
-
*[[Nucleoside diphosphate kinase|Nucleoside diphosphate kinase]]
+
*[[Nucleoside diphosphate kinase 3D structures|Nucleoside diphosphate kinase 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:011277918</ref><references group="xtra"/>
+
__TOC__
 +
</StructureSection>
[[Category: Dictyostelium discoideum]]
[[Category: Dictyostelium discoideum]]
-
[[Category: Nucleoside-diphosphate kinase]]
+
[[Category: Large Structures]]
-
[[Category: Babolat, M.]]
+
[[Category: Babolat M]]
-
[[Category: Deville-Bonne, D.]]
+
[[Category: Deville-Bonne D]]
-
[[Category: Janin, J.]]
+
[[Category: Janin J]]
-
[[Category: Schneider, B.]]
+
[[Category: Schneider B]]
-
[[Category: Veron, M.]]
+
[[Category: Veron M]]
-
[[Category: Xu, Y W.]]
+
[[Category: Xu YW]]
-
[[Category: Kinase]]
+
-
[[Category: Metabolic role]]
+
-
[[Category: Transferase]]
+

Current revision

Role of active site resiude Lys16 in Nucleoside Diphosphate Kinase

PDB ID 1hhq

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1hhq"
Personal tools