1qln

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[[Image:1qln.png|left|200px]]
 
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==STRUCTURE OF A TRANSCRIBING T7 RNA POLYMERASE INITIATION COMPLEX==
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The line below this paragraph, containing "STRUCTURE_1qln", creates the "Structure Box" on the page.
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<StructureSection load='1qln' size='340' side='right'caption='[[1qln]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1qln]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QLN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QLN FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>
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{{STRUCTURE_1qln| PDB=1qln | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qln OCA], [https://pdbe.org/1qln PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qln RCSB], [https://www.ebi.ac.uk/pdbsum/1qln PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qln ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPOL_BPT7 RPOL_BPT7] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of the late genes of T7. It is rifampicin-resistant. It recognizes a specific promoter sequence, unwinds the double-stranded RNA to expose the coding strand for templating, initiates transcription preferentially with a purine.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ql/1qln_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qln ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of a T7 RNA polymerase (T7 RNAP) initiation complex captured transcribing a trinucleotide of RNA from a 17-base pair promoter DNA containing a 5-nucleotide single-strand template extension was determined at a resolution of 2.4 angstroms. Binding of the upstream duplex portion of the promoter occurs in the same manner as that in the open promoter complex, but the single-stranded template is repositioned to place the +4 base at the catalytic active site. Thus, synthesis of RNA in the initiation phase leads to accumulation or "scrunching" of the template in the enclosed active site pocket of T7 RNAP. Only three base pairs of heteroduplex are formed before the RNA peels off the template.
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===STRUCTURE OF A TRANSCRIBING T7 RNA POLYMERASE INITIATION COMPLEX===
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Structure of a transcribing T7 RNA polymerase initiation complex.,Cheetham GM, Steitz TA Science. 1999 Dec 17;286(5448):2305-9. PMID:10600732<ref>PMID:10600732</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_10600732}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1qln" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 10600732 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10600732}}
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==About this Structure==
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[[1qln]] is a 4 chain structure of [[Plant Viral Protein p19 Suppression of RNA Silencing]] and [[RNA polymerase]] with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QLN OCA].
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==See Also==
==See Also==
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*[[Molecular Playground/Human mtRNA pol|Molecular Playground/Human mtRNA pol]]
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*[[P19|P19]]
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*[[Molecular Playground/T7 RNA Polymerase (7 mer int)|Molecular Playground/T7 RNA Polymerase (7 mer int)]]
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*[[Molecular Playground/T7 RNAP Conformations|Molecular Playground/T7 RNAP Conformations]]
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*[[Plant Viral Protein p19 Suppression of RNA Silencing|Plant Viral Protein p19 Suppression of RNA Silencing]]
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*[[RNA polymerase|RNA polymerase]]
*[[RNA polymerase|RNA polymerase]]
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*[[User:Luis E Ramirez-Tapia/T7 RNA polymerase|User:Luis E Ramirez-Tapia/T7 RNA polymerase]]
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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*[[User:Wayne Decatur/Plant Viral Protein p19 Suppression of RNA Silencing|User:Wayne Decatur/Plant Viral Protein p19 Suppression of RNA Silencing]]
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:010600732</ref><references group="xtra"/>
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</StructureSection>
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[[Category: DNA-directed RNA polymerase]]
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[[Category: Escherichia phage T7]]
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[[Category: Enterobacteria phage t7]]
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[[Category: Large Structures]]
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[[Category: Cheetham, G M.T.]]
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[[Category: Cheetham GMT]]
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[[Category: Steitz, T A.]]
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[[Category: Steitz TA]]
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[[Category: Nucleotidyltransferase]]
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[[Category: Protein/dna/rna]]
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[[Category: T7 rna polymerase]]
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Current revision

STRUCTURE OF A TRANSCRIBING T7 RNA POLYMERASE INITIATION COMPLEX

PDB ID 1qln

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