4apb

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[[Image:4apb.png|left|200px]]
 
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==Crystal structure of Mycobacterium tuberculosis fumarase (Rv1098c) S318C in complex with fumarate==
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The line below this paragraph, containing "STRUCTURE_4apb", creates the "Structure Box" on the page.
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<StructureSection load='4apb' size='340' side='right'caption='[[4apb]], [[Resolution|resolution]] 1.94&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[4apb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4APB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4APB FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene></td></tr>
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{{STRUCTURE_4apb| PDB=4apb | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4apb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4apb OCA], [https://pdbe.org/4apb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4apb RCSB], [https://www.ebi.ac.uk/pdbsum/4apb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4apb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FUMC_MYCTU FUMC_MYCTU] Catalyzes the reversible addition of water to fumarate to give L-malate.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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rv1098c, an essential gene in Mycobacterium tuberculosis, codes for a class II fumarase. We describe here the crystal structure of Rv1098c in complex with l-malate, fumarate or the competitive inhibitor meso-tartrate. The models reveal that substrate binding promotes the closure of the active site through conformational changes involving the catalytic SS-loop and the C-terminal domain, which likely represents a general feature of this enzyme superfamily. Analysis of ligand-enzyme interactions as well as site-directed mutagenesis suggest Ser318 as one of the two acid-base catalysts. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: Rv1098c and Rv1098c bind by X-ray crystallography (View interaction).
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===Crystal structure of Mycobacterium tuberculosis fumarase (Rv1098c) S318C in complex with fumarate===
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Conformational changes upon ligand binding in the essential class II fumarase Rv1098c from Mycobacterium tuberculosis.,Mechaly AE, Haouz A, Miras I, Barilone N, Weber P, Shepard W, Alzari PM, Bellinzoni M FEBS Lett. 2012 Jun 4;586(11):1606-11. Epub 2012 May 3. PMID:22561013<ref>PMID:22561013</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4apb" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_22561013}}, adds the Publication Abstract to the page
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*[[Fumarase|Fumarase]]
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(as it appears on PubMed at http://www.pubmed.gov), where 22561013 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_22561013}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[4apb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4APB OCA].
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Alzari PM]]
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==Reference==
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[[Category: Bellinzoni M]]
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<ref group="xtra">PMID:022561013</ref><references group="xtra"/>
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[[Category: Haouz A]]
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[[Category: Fumarate hydratase]]
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[[Category: Mechaly AE]]
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Alzari, P M.]]
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[[Category: Bellinzoni, M.]]
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[[Category: Haouz, A.]]
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[[Category: Mechaly, A E.]]
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[[Category: Hydrolase]]
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[[Category: Metabolism]]
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Current revision

Crystal structure of Mycobacterium tuberculosis fumarase (Rv1098c) S318C in complex with fumarate

PDB ID 4apb

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